Event Title

Session D-6: Green Fluorescent Protein

Session Number

D-6

Location

A206

Grade Level

Grade Level: 9-12

Start Date

25-2-2011 1:00 PM

End Date

24-2-2011 2:00 PM

Description

GFP purification requires four major steps: inoculation of colonies and growing the cell culture; lysis of cell membrane to extract the protein; purification of protein using hydrophobic column; and use of SDS electrophoresis gel to check for purity. Stations will be set up for you to experience these steps. You can use this experience to adapt this lab in your own classroom or teach students about biotechnology.

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Feb 25th, 1:00 PM Feb 24th, 2:00 PM

Session D-6: Green Fluorescent Protein

A206

GFP purification requires four major steps: inoculation of colonies and growing the cell culture; lysis of cell membrane to extract the protein; purification of protein using hydrophobic column; and use of SDS electrophoresis gel to check for purity. Stations will be set up for you to experience these steps. You can use this experience to adapt this lab in your own classroom or teach students about biotechnology.