Event Title

Localizing GABAergic/Dopaminergic Hybrid Neurons

Session Number

Q09

Advisor(s)

Rajeshwar Awatramani, Northwestern University
Giuliana Caronia-Brown, Northwestern University
Jean-Francois Poulin, Northwestern University

Location

A-133

Start Date

28-4-2016 8:25 AM

End Date

28-4-2016 8:50 AM

Disciplines

Neuroscience and Neurobiology

Abstract

Dopaminergic (DA) neurons are the primary source of the dopamine produced in the central nervous system. These DA neurons have recently been shown to have diverse molecular and physiological properties. A recent study implied that the SNc neurons may release the neurotransmitter, GABA, along with dopamine. Through our research, we aimed to localize these “hybrid” neurons which produce both GABA and dopamine together. To achieve this objective we utilized an intersectional approach. We crossed the VgatCre mouse line to a ThFlpo,Frepe mouse line. The first mouse line expresses Cre recombinase from Vesicular GABA transporter (Vgat), a gene that defines GABAergic neurons. The latter mouse line allows expression of Flpo recombinase only in DA neurons. When the VgatCre and ThFlpo gene are both expressed, the reporter gene expresses a green fluorescent protein (GFP), and this allows us to localize these “hybrid” DA neurons. The results showed 11% of SNc dopamine neurons as hybrid neurons. On the other hand, 19% of VTA dopamine neurons are shown as hybrid neurons. The results reveal a higher density of hybrid neurons in the VTA than in SNc. Further studies will be needed to determine the functional consequences of Vgat in DA neurons


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Apr 28th, 8:25 AM Apr 28th, 8:50 AM

Localizing GABAergic/Dopaminergic Hybrid Neurons

A-133

Dopaminergic (DA) neurons are the primary source of the dopamine produced in the central nervous system. These DA neurons have recently been shown to have diverse molecular and physiological properties. A recent study implied that the SNc neurons may release the neurotransmitter, GABA, along with dopamine. Through our research, we aimed to localize these “hybrid” neurons which produce both GABA and dopamine together. To achieve this objective we utilized an intersectional approach. We crossed the VgatCre mouse line to a ThFlpo,Frepe mouse line. The first mouse line expresses Cre recombinase from Vesicular GABA transporter (Vgat), a gene that defines GABAergic neurons. The latter mouse line allows expression of Flpo recombinase only in DA neurons. When the VgatCre and ThFlpo gene are both expressed, the reporter gene expresses a green fluorescent protein (GFP), and this allows us to localize these “hybrid” DA neurons. The results showed 11% of SNc dopamine neurons as hybrid neurons. On the other hand, 19% of VTA dopamine neurons are shown as hybrid neurons. The results reveal a higher density of hybrid neurons in the VTA than in SNc. Further studies will be needed to determine the functional consequences of Vgat in DA neurons