Session Number

C867

Advisor(s)

Joanna Jelenska, University of Chicago

Location

B-206 Lecture Hall

Start Date

28-4-2016 10:40 AM

End Date

28-4-2016 11:05 AM

Disciplines

Biology

Abstract

In order to study the plant immune system for benefits in crop production, a protocol was developed to effectively transform the 76R variety Rio-Grande tomato to express the HopZ3 effector protein which is found in Pseudomonas syringae, a bacteria which is pathogenic to plants. Cotyledons from the 76R variety were infected with Agrobacteria to insert HopZ3 bacterial genes into plant cells, and these cotyledons were placed in media with a selection agent and hormones to induce callous growth, plantlet growth, shoot growth, and root growth until only transformed cells remain. In the most successful test thus far, cotyledons have successfully been transformed, but do not survive in the media used for more than 4-5 weeks, and different Agrobacteria strains and hormone concentrations are being tested. If successful, the tomato plant would express the HopZ3 protein which would be further studied for its role in immune system pathways in the tomato plant cell.

Included in

Biology Commons

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Apr 28th, 10:40 AM Apr 28th, 11:05 AM

Factors Affecting the Transformation of 76R Variety Rio-Grande Tomatoes to Induce Expression of HopZ3 Effector Protein

B-206 Lecture Hall

In order to study the plant immune system for benefits in crop production, a protocol was developed to effectively transform the 76R variety Rio-Grande tomato to express the HopZ3 effector protein which is found in Pseudomonas syringae, a bacteria which is pathogenic to plants. Cotyledons from the 76R variety were infected with Agrobacteria to insert HopZ3 bacterial genes into plant cells, and these cotyledons were placed in media with a selection agent and hormones to induce callous growth, plantlet growth, shoot growth, and root growth until only transformed cells remain. In the most successful test thus far, cotyledons have successfully been transformed, but do not survive in the media used for more than 4-5 weeks, and different Agrobacteria strains and hormone concentrations are being tested. If successful, the tomato plant would express the HopZ3 protein which would be further studied for its role in immune system pathways in the tomato plant cell.

 

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