Event Title

Effect of Hormones on Breast Cancer Cell Line Enhancers

Session Number

C27

Advisor(s)

Michael Bolt, University of Chicago
Heather Scott, University of Chicago
Kevin White, University of Chicago

Location

B-131 Grainger

Start Date

28-4-2016 1:35 PM

End Date

28-4-2016 2:00 PM

Disciplines

Biology

Abstract

Breast cancer pervades society, so its cell lines are studied to find therapies. Hormones and enhancer regions play a central role in messenger ribonucleic acid (mRNA) levels of genes, so observing them can help develop new therapies. We use a luminal A breast cancer cell line model, MCF-7, to determine the effect of different hormones on gene expression. Hormone effect on gene expression within cell lines needed to be determined by Quantitative Polymerase Chain Reaction (qPCR), a process that determines the expression levels of ribonucleic acids of specific gene targets. Preliminary results suggest each MCF- 7 ligand used (E2, Dexamethasone, GW0742, AM580, T3, and Rosiglitazone) showed hormone specific effects in gene expression. T3 and ROSI both primarily up-regulate their targets, while the remaining hormones are more of a mix of up and down regulated genes. However, future studies will have to confirm this using mRNA sequencing to determine the genome-wide transcription effects of these hormone. CapStarr-seq (self-transcribing active regulatory region sequencing) will be used to determine enhancers involved. By studying the interactions of hormones on MCF-7, we hope to further understand cell line interactions in breast cancer for greater therapeutic options in patients and understand this form of breast cancer better.


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Apr 28th, 1:35 PM Apr 28th, 2:00 PM

Effect of Hormones on Breast Cancer Cell Line Enhancers

B-131 Grainger

Breast cancer pervades society, so its cell lines are studied to find therapies. Hormones and enhancer regions play a central role in messenger ribonucleic acid (mRNA) levels of genes, so observing them can help develop new therapies. We use a luminal A breast cancer cell line model, MCF-7, to determine the effect of different hormones on gene expression. Hormone effect on gene expression within cell lines needed to be determined by Quantitative Polymerase Chain Reaction (qPCR), a process that determines the expression levels of ribonucleic acids of specific gene targets. Preliminary results suggest each MCF- 7 ligand used (E2, Dexamethasone, GW0742, AM580, T3, and Rosiglitazone) showed hormone specific effects in gene expression. T3 and ROSI both primarily up-regulate their targets, while the remaining hormones are more of a mix of up and down regulated genes. However, future studies will have to confirm this using mRNA sequencing to determine the genome-wide transcription effects of these hormone. CapStarr-seq (self-transcribing active regulatory region sequencing) will be used to determine enhancers involved. By studying the interactions of hormones on MCF-7, we hope to further understand cell line interactions in breast cancer for greater therapeutic options in patients and understand this form of breast cancer better.