Identifying Toxic Amyloid-Beta Oligomers Species (AβOs) in Alzheimer’s disease
Session Number
Q25
Advisor(s)
Erika Cline, Northwestern University William Klein, Northwestern University Kirsten Viola, Northwestern University
Location
A-123
Start Date
28-4-2016 2:00 PM
End Date
28-4-2016 2:25 PM
Abstract
In the field of neurobiology, there is great discussion about which particular species of amyloid-beta oligomers (AβOs) contributes to the pathogenesis of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Previously, our group has found, using molecular weight cutoff filters (MWCO), that AβOs are primarily 100-300 kilodaltons (kDa). The goal of this project is to verify this finding using size exclusion chromatography (SEC). SEC can be used to determine AβO molecular weight according to the amount of time it takes the AβOs to pass through the SEC column. Overall, the project yielded results showing significant portion of AβOs are either >1300 kDa or around 13 kDa. We are currently investigating further reasons why the MWCO and SEC data yield very different results.
Identifying Toxic Amyloid-Beta Oligomers Species (AβOs) in Alzheimer’s disease
A-123
In the field of neurobiology, there is great discussion about which particular species of amyloid-beta oligomers (AβOs) contributes to the pathogenesis of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Previously, our group has found, using molecular weight cutoff filters (MWCO), that AβOs are primarily 100-300 kilodaltons (kDa). The goal of this project is to verify this finding using size exclusion chromatography (SEC). SEC can be used to determine AβO molecular weight according to the amount of time it takes the AβOs to pass through the SEC column. Overall, the project yielded results showing significant portion of AβOs are either >1300 kDa or around 13 kDa. We are currently investigating further reasons why the MWCO and SEC data yield very different results.