Event Title

Identifying Toxic Amyloid-Beta Oligomers Species (AβOs) in Alzheimer’s disease

Session Number

Q25

Advisor(s)

Erika Cline, Northwestern University
William Klein, Northwestern University
Kirsten Viola, Northwestern University

Location

A-123

Start Date

28-4-2016 2:00 PM

End Date

28-4-2016 2:25 PM

Disciplines

Neuroscience and Neurobiology

Abstract

In the field of neurobiology, there is great discussion about which particular species of amyloid-beta oligomers (AβOs) contributes to the pathogenesis of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Previously, our group has found, using molecular weight cutoff filters (MWCO), that AβOs are primarily 100-300 kilodaltons (kDa). The goal of this project is to verify this finding using size exclusion chromatography (SEC). SEC can be used to determine AβO molecular weight according to the amount of time it takes the AβOs to pass through the SEC column. Overall, the project yielded results showing significant portion of AβOs are either >1300 kDa or around 13 kDa. We are currently investigating further reasons why the MWCO and SEC data yield very different results.


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Apr 28th, 2:00 PM Apr 28th, 2:25 PM

Identifying Toxic Amyloid-Beta Oligomers Species (AβOs) in Alzheimer’s disease

A-123

In the field of neurobiology, there is great discussion about which particular species of amyloid-beta oligomers (AβOs) contributes to the pathogenesis of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Previously, our group has found, using molecular weight cutoff filters (MWCO), that AβOs are primarily 100-300 kilodaltons (kDa). The goal of this project is to verify this finding using size exclusion chromatography (SEC). SEC can be used to determine AβO molecular weight according to the amount of time it takes the AβOs to pass through the SEC column. Overall, the project yielded results showing significant portion of AβOs are either >1300 kDa or around 13 kDa. We are currently investigating further reasons why the MWCO and SEC data yield very different results.