Event Title

The Effects of the Ndc80 Complex on the Kinetochore Attachment to Microtubules

Advisor(s)

Dr. Dileep Varma, Feinberg School of Medicine

Location

Room A121

Start Date

26-4-2019 10:05 AM

End Date

26-4-2019 10:20 AM

Abstract

In past years, several arguments have been made about the molecules that affect chromosome separation. This SIR investigation focused on the recent advances in primary interface between the kinetochore and kMTs. Prior research has shown that proper mitotic chromosome alignment and separation require kinetochore-microtubule attachments, which are mediated by Ndc80, a kMT binding complex, as well as the molecular motor dynein. The Rod-ZW10-Zwilch complex is crucial to the coordination between these complexes and kMT attachments because it has an essential role in dynein recruitment and activation of the spindle assembly checkpoint. The project focused on learning the mechanism by which protein complexes control kMT attachments in order to drive the chromosome motility during early mitosis. The experimenters used in vitro total internal reflection fluorescence microscopy to observe the concentrations of Ndc80 inhibited dynein function. They observed that the higher the concentration of Ndc80, the higher the levels of inhibited dynein function. This result provided evidence that Ndc80 negatively affects the function of the molecular motor dynein.

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Apr 26th, 10:05 AM Apr 26th, 10:20 AM

The Effects of the Ndc80 Complex on the Kinetochore Attachment to Microtubules

Room A121

In past years, several arguments have been made about the molecules that affect chromosome separation. This SIR investigation focused on the recent advances in primary interface between the kinetochore and kMTs. Prior research has shown that proper mitotic chromosome alignment and separation require kinetochore-microtubule attachments, which are mediated by Ndc80, a kMT binding complex, as well as the molecular motor dynein. The Rod-ZW10-Zwilch complex is crucial to the coordination between these complexes and kMT attachments because it has an essential role in dynein recruitment and activation of the spindle assembly checkpoint. The project focused on learning the mechanism by which protein complexes control kMT attachments in order to drive the chromosome motility during early mitosis. The experimenters used in vitro total internal reflection fluorescence microscopy to observe the concentrations of Ndc80 inhibited dynein function. They observed that the higher the concentration of Ndc80, the higher the levels of inhibited dynein function. This result provided evidence that Ndc80 negatively affects the function of the molecular motor dynein.