Event Title

Nucleolar Impact on KeratinExploringocyte Differentiation

Advisor(s)

Dr. Sui Huang

Location

Room A113

Start Date

26-4-2019 11:25 AM

End Date

26-4-2019 11:40 AM

Abstract

As the largest components within the nucleus, nucleoli consist of ribosomal DNA regions and are essential for ribosome synthesis. Yet, for such distinct regions within the central component of cells, there has been little research concerning its influence on cellular metabolism outside of ribosome synthesis. Thus, nucleoli may play key roles in crucial cell processes, including involvement with cellular differentiation. In this study, we observed the change in nucleolar count as keratinocytes differentiate, measuring other factors across epidermal layers including the quantity of cells without nucleoli, centromere count, centromere association with the nucleolus, and centromere association with the nuclear membrane. By defining layers based on general distance from the basal cell layer, we detected correlations between cells of different levels of differentiation for the aforementioned factors. These significant changes across layers suggest that the nucleolus plays an active role in differentiation, promoting further investigation into the nucleolus’ holistic function.

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Apr 26th, 11:25 AM Apr 26th, 11:40 AM

Nucleolar Impact on KeratinExploringocyte Differentiation

Room A113

As the largest components within the nucleus, nucleoli consist of ribosomal DNA regions and are essential for ribosome synthesis. Yet, for such distinct regions within the central component of cells, there has been little research concerning its influence on cellular metabolism outside of ribosome synthesis. Thus, nucleoli may play key roles in crucial cell processes, including involvement with cellular differentiation. In this study, we observed the change in nucleolar count as keratinocytes differentiate, measuring other factors across epidermal layers including the quantity of cells without nucleoli, centromere count, centromere association with the nucleolus, and centromere association with the nuclear membrane. By defining layers based on general distance from the basal cell layer, we detected correlations between cells of different levels of differentiation for the aforementioned factors. These significant changes across layers suggest that the nucleolus plays an active role in differentiation, promoting further investigation into the nucleolus’ holistic function.