Event Title

Cell Proliferation Of Glioblastoma Depending On Age Using TORC1 Enzymes

Session Number

Project ID: MEDH 18

Advisor(s)

Dr. Sowmya Anjur; Illinois Mathematics and Science Academy

Discipline

Medical and Health Sciences

Start Date

22-4-2020 8:30 AM

End Date

22-4-2020 8:45 AM

Abstract

Using three Glioblastoma cell lines; T89G, U118, U87, we looked at how fast the glioblastoma spread based on the age of the person each cell strand belonged to. We also looked at how present the TORC1 enzyme, which helps regulate cell growth, was in each cell strand to see if there was a correlation with the cell proliferation. We grew each cell line in flasks and maintained them by changing out their media, so they could grow to a point where we had enough cells to work with. We used the ELISA assay to see how much of the TORC1 enzyme is in each of the cell lines. After we used the ELISA assay, we compared the concentrations of the TORC1 enzymes in each cell line to the actual concentration in the rabbit TORC1. We also used a Matrix assay to compare cell proliferation and see if there were any other proteins that had a correlation with the cell proliferation.

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Apr 22nd, 8:30 AM Apr 22nd, 8:45 AM

Cell Proliferation Of Glioblastoma Depending On Age Using TORC1 Enzymes

Using three Glioblastoma cell lines; T89G, U118, U87, we looked at how fast the glioblastoma spread based on the age of the person each cell strand belonged to. We also looked at how present the TORC1 enzyme, which helps regulate cell growth, was in each cell strand to see if there was a correlation with the cell proliferation. We grew each cell line in flasks and maintained them by changing out their media, so they could grow to a point where we had enough cells to work with. We used the ELISA assay to see how much of the TORC1 enzyme is in each of the cell lines. After we used the ELISA assay, we compared the concentrations of the TORC1 enzymes in each cell line to the actual concentration in the rabbit TORC1. We also used a Matrix assay to compare cell proliferation and see if there were any other proteins that had a correlation with the cell proliferation.