Event Title

Detection of mutagenic agent MDA in human ovaries

Session Number

Project ID: MEDH 24

Advisor(s)

Dr. Animesh Barua; Rush University, Chicago

Discipline

Medical and Health Sciences

Start Date

22-4-2020 8:50 AM

End Date

22-4-2020 9:05 AM

Abstract

Defective DNA-damage repair mechanism together with formation of mutagenic DNA adducts (a portion of DNA bound with cancer causing agent) is a hallmark of cancer development. Malondialdehyde (MDA) is one of such agents produced during lipid peroxidation, can form mutagenic DNA adducts and may be a marker of early detection of ovarian cancer (OVCA), a lethal disease of women. The goal of this pilot study was to determine the changes in MDA expression in ovaries in women during aging. Ovarian specimens were collected from women of reproductive age (<50 >years, n=5), postmenopausal women (60-65 years old, n=5) and women with OVCA at early stage (n=5). Expression of MDA was determined by immunohistochemistry, protein and gene expression studies. Significant differences in the intensities of MDA expression among different groups were assessed by ANOVA and paired t-tests and significance was taken when P

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Apr 22nd, 8:50 AM Apr 22nd, 9:05 AM

Detection of mutagenic agent MDA in human ovaries

Defective DNA-damage repair mechanism together with formation of mutagenic DNA adducts (a portion of DNA bound with cancer causing agent) is a hallmark of cancer development. Malondialdehyde (MDA) is one of such agents produced during lipid peroxidation, can form mutagenic DNA adducts and may be a marker of early detection of ovarian cancer (OVCA), a lethal disease of women. The goal of this pilot study was to determine the changes in MDA expression in ovaries in women during aging. Ovarian specimens were collected from women of reproductive age (<50>years, n=5), postmenopausal women (60-65 years old, n=5) and women with OVCA at early stage (n=5). Expression of MDA was determined by immunohistochemistry, protein and gene expression studies. Significant differences in the intensities of MDA expression among different groups were assessed by ANOVA and paired t-tests and significance was taken when P