Event Title

The Effect of Upregulation of the Canonical Wnt Signaling Pathway on Intramembranous Bone Regeneration

Session Number

Project ID: BIO 38

Advisor(s)

Dr. Frank Ko; Rush University Medical Center

Discipline

Biology

Start Date

22-4-2020 9:10 AM

End Date

4-2020 9:25 AM

Abstract

Background: Proper intramembranous bone regeneration is critical for the longevity of orthopaedic implants. It is currently known that the upregulation of the canonical Wnt signaling pathway results in the high bone mass (HBM) phenotype and faster healing of fractures. The goal of this study is to determine whether the upregulation of the canonical Wnt pathway also leads to faster intramembranous bone regeneration following bone marrow ablation surgery.

Methods: Both HBM mice expressing higher levels of Wnt signaling and wild type (WT) mice were used. All mice underwent unilateral femoral bone marrow ablation surgery at 4 weeks old and recovered for 14 days. Histology by H&E staining and regenerated BV/TV by microCT were assessed.

Results: We found that a significant difference in BV/TV values exists between HBM females and WT females, and WT females had a 63% lower bone density than HBM females (p = 0.011). No significant difference exists between HBM males and WT males.

Conclusions: This suggests that the upregulation of the canonical Wnt pathway leads to a faster rate of bone regeneration following bone marrow ablation surgery in female mice. These findings also imply that artificially upregulating Wnt signaling in bone surgery patients may lead to a faster recovery time.

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Apr 22nd, 9:10 AM Apr 1st, 9:25 AM

The Effect of Upregulation of the Canonical Wnt Signaling Pathway on Intramembranous Bone Regeneration

Background: Proper intramembranous bone regeneration is critical for the longevity of orthopaedic implants. It is currently known that the upregulation of the canonical Wnt signaling pathway results in the high bone mass (HBM) phenotype and faster healing of fractures. The goal of this study is to determine whether the upregulation of the canonical Wnt pathway also leads to faster intramembranous bone regeneration following bone marrow ablation surgery.

Methods: Both HBM mice expressing higher levels of Wnt signaling and wild type (WT) mice were used. All mice underwent unilateral femoral bone marrow ablation surgery at 4 weeks old and recovered for 14 days. Histology by H&E staining and regenerated BV/TV by microCT were assessed.

Results: We found that a significant difference in BV/TV values exists between HBM females and WT females, and WT females had a 63% lower bone density than HBM females (p = 0.011). No significant difference exists between HBM males and WT males.

Conclusions: This suggests that the upregulation of the canonical Wnt pathway leads to a faster rate of bone regeneration following bone marrow ablation surgery in female mice. These findings also imply that artificially upregulating Wnt signaling in bone surgery patients may lead to a faster recovery time.