Event Title

Investigating the Role of TET2 Enzymes in the Proliferation, Differentiation, and Survival of Hematopoietic Stem Cells

Session Number

Project ID: BIO 08

Advisor(s)

Dr. Vipul Shukla; Northwestern Univeristy, Feinberg School of Medicine

Dr. Ajay Abraham; Northwestern Univeristy, Feinberg School of Medicine

Discipline

Biology

Start Date

19-4-2023 10:50 AM

End Date

19-4-2023 11:05 AM

Abstract

Ten-Eleven Translocation (TET) enzymes play a critical role in DNA demethylation, which is essential for mammalian development and differentiation. Recent research suggests that the absence of TET enzymes is associated with an increased risk of malignancies. Among the TET enzymes, TET2 is the most commonly mutated and the focus of our investigation. Using gene editing techniques based on clustered regularly interspaced short palindromic repeat (CRISPR), we aim to study the impact of TET enzyme removal from hematopoietic stem cells in mice. Our study builds on the findings of Dr. Vipul Shukla, whose research has shown that TET deficiency disrupts mature B cell homeostasis, leading to oncogenesis characterized by the accumulation of G-quadruplex and R-loop structures. Through our work, we hope to gain a deeper understanding of the role TET enzymes play in the proliferation, differentiation, and survival of hematopoietic stem cells in mice and identify potential therapeutic strategies.

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Apr 19th, 10:50 AM Apr 19th, 11:05 AM

Investigating the Role of TET2 Enzymes in the Proliferation, Differentiation, and Survival of Hematopoietic Stem Cells

Ten-Eleven Translocation (TET) enzymes play a critical role in DNA demethylation, which is essential for mammalian development and differentiation. Recent research suggests that the absence of TET enzymes is associated with an increased risk of malignancies. Among the TET enzymes, TET2 is the most commonly mutated and the focus of our investigation. Using gene editing techniques based on clustered regularly interspaced short palindromic repeat (CRISPR), we aim to study the impact of TET enzyme removal from hematopoietic stem cells in mice. Our study builds on the findings of Dr. Vipul Shukla, whose research has shown that TET deficiency disrupts mature B cell homeostasis, leading to oncogenesis characterized by the accumulation of G-quadruplex and R-loop structures. Through our work, we hope to gain a deeper understanding of the role TET enzymes play in the proliferation, differentiation, and survival of hematopoietic stem cells in mice and identify potential therapeutic strategies.