Event Title

The Cytotoxic Effect of Melittin and TsAP 2 on pBR322 Vector DNA

Session Number

B11

Advisor(s)

Santosh Misra, Carle Biomedical Research Center
Dipanjan Pan, University of Illinois at Urbana-Champaign

Location

B-115

Start Date

28-4-2016 8:00 AM

End Date

28-4-2016 8:25 AM

Disciplines

Biomedical Engineering and Bioengineering

Abstract

The instability of genomic deoxyribonucleic acid (DNA) plays an integral role in carcinogenesis and metastasis by causing mutations that regulate cell division and expression. Degrading the genomic DNA that governs the cell cycle will downregulate both proliferation and differentiation. In an attempt to discover therapeutic agents that would distort the molecular structure of DNA, this investigation uncovered medicinal effects of the cytotoxic and amphipathic peptides, melittin and TsAP 2, on pBR322 Vector DNA. Gel electrophoresis and ultraviolet imaging allowed us to determine the optimal concentration range of melittin and tityusserrulatus antimicrobial peptide (TsAP 2) needed to distort the charge of DNA. In each trial, the ratio of DNA to peptide assorted from 1:0.25 to 1:2.5, respectively. Preliminary gel images suggest that as the peptide concentration increased, the amount of unbound DNA decreased. Specifically, melittin is able to bind and distort DNA at a concentration of 25 micromolar (μM) while TsAP 2 is able to do so at a concentration of 150 μM. I speculated that the peptides caused long- range electrostatic interactions in the backbone of DNA, causing the helix to condense and collapse, I concluded that melittin and TsAP 2 are potent distorters of DNA.


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Apr 28th, 8:00 AM Apr 28th, 8:25 AM

The Cytotoxic Effect of Melittin and TsAP 2 on pBR322 Vector DNA

B-115

The instability of genomic deoxyribonucleic acid (DNA) plays an integral role in carcinogenesis and metastasis by causing mutations that regulate cell division and expression. Degrading the genomic DNA that governs the cell cycle will downregulate both proliferation and differentiation. In an attempt to discover therapeutic agents that would distort the molecular structure of DNA, this investigation uncovered medicinal effects of the cytotoxic and amphipathic peptides, melittin and TsAP 2, on pBR322 Vector DNA. Gel electrophoresis and ultraviolet imaging allowed us to determine the optimal concentration range of melittin and tityusserrulatus antimicrobial peptide (TsAP 2) needed to distort the charge of DNA. In each trial, the ratio of DNA to peptide assorted from 1:0.25 to 1:2.5, respectively. Preliminary gel images suggest that as the peptide concentration increased, the amount of unbound DNA decreased. Specifically, melittin is able to bind and distort DNA at a concentration of 25 micromolar (μM) while TsAP 2 is able to do so at a concentration of 150 μM. I speculated that the peptides caused long- range electrostatic interactions in the backbone of DNA, causing the helix to condense and collapse, I concluded that melittin and TsAP 2 are potent distorters of DNA.