Event Title

The Effect of Different Concentrations of Camptothecin on the Apoptosis of MCF-7, U937, and CEM Cancer Cells

Session Number

C09

Advisor(s)

Don Dosch, Illinois Mathematics and Science Academy

Location

A-113

Start Date

28-4-2016 8:50 AM

End Date

28-4-2016 9:15 AM

Disciplines

Biology

Abstract

Camptothecin has been found to inhibit topoisomerase 1, which is essential for DNA replication. With a decreased amount of topoisomerase 1, there is an excess of reversible cleaving complexes which initiates apoptosis. Through microscope observation of camptothecin treated cells, concentrations of camptothecin were narrowed down in order to determine a range of 0.2 micromolar (uM) to 0.8 uM to use when running enzyme-linked immunosorbent assay (ELISA) tests. The ELISA test indicates the level of cleaved caspase 3, which is present in higher amounts when apoptosis has occurred, indicating that a camptothecin concentration has affected the cancer cells. The ELISA tests were run with camptothecin concentrations in the previously determined range, and the results provide a starting point for further research into investigating if camptothecin is a viable option for cancer treatment.


Share

COinS
 
Apr 28th, 8:50 AM Apr 28th, 9:15 AM

The Effect of Different Concentrations of Camptothecin on the Apoptosis of MCF-7, U937, and CEM Cancer Cells

A-113

Camptothecin has been found to inhibit topoisomerase 1, which is essential for DNA replication. With a decreased amount of topoisomerase 1, there is an excess of reversible cleaving complexes which initiates apoptosis. Through microscope observation of camptothecin treated cells, concentrations of camptothecin were narrowed down in order to determine a range of 0.2 micromolar (uM) to 0.8 uM to use when running enzyme-linked immunosorbent assay (ELISA) tests. The ELISA test indicates the level of cleaved caspase 3, which is present in higher amounts when apoptosis has occurred, indicating that a camptothecin concentration has affected the cancer cells. The ELISA tests were run with camptothecin concentrations in the previously determined range, and the results provide a starting point for further research into investigating if camptothecin is a viable option for cancer treatment.