Event Title

TIM4 detection in Hepatic Stellate Cells

Session Number

C31

Advisor(s)

Xiao Wang, Northwestern University

Location

B-110

Start Date

28-4-2016 9:15 AM

End Date

28-4-2016 9:40 AM

Disciplines

Biology

Abstract

HSC (hepatic stellate cell) activation has emerged as a key player in liver injury and fibrosis development. However, the molecules and signal pathways involved in liver fibrosis are unclear. The aim of this study is to identify the molecule related to HSC phagocytosis and activation. TIM4 is a cell receptor used by activated T-cells and could potentially be a mediator for HSC phagocytosis. We determined TIM4 RNA expression by real-time PCR (polymerase chain reaction) and protein level by western blot, respectively. We found that both TIM4 RNA and protein are expressed at low levels, suggesting that TIM4 may not play a major role. It would be interesting to explore if inflammation or other stress can induce TIM4 expression in HSCs and if the increase in TIM4 can be attributed to HSC activation in liver diseases. Identifying such a biomarker would have potential for clinical use.


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Apr 28th, 9:15 AM Apr 28th, 9:40 AM

TIM4 detection in Hepatic Stellate Cells

B-110

HSC (hepatic stellate cell) activation has emerged as a key player in liver injury and fibrosis development. However, the molecules and signal pathways involved in liver fibrosis are unclear. The aim of this study is to identify the molecule related to HSC phagocytosis and activation. TIM4 is a cell receptor used by activated T-cells and could potentially be a mediator for HSC phagocytosis. We determined TIM4 RNA expression by real-time PCR (polymerase chain reaction) and protein level by western blot, respectively. We found that both TIM4 RNA and protein are expressed at low levels, suggesting that TIM4 may not play a major role. It would be interesting to explore if inflammation or other stress can induce TIM4 expression in HSCs and if the increase in TIM4 can be attributed to HSC activation in liver diseases. Identifying such a biomarker would have potential for clinical use.