Event Title

Session 1I: Electrochemical Detection of Alpha-Melanocyte Stimulating Hormone Using Flow Injection Analysis with Amperometric Detection

Session Number

Session 1I: 3rd Presentation

Advisor(s)

Greg Swain, Michigan State University

Location

Room B101

Start Date

28-4-2017 8:30 AM

End Date

28-4-2017 9:45 AM

Abstract

The detection of pharmaceutical compounds, biomolecules, and chemicals plays a critical role in medical care, drug development, and environmental testing. In this study, the analytical performances of two novel materials, boron-doped nanocrystalline diamond (BDD) and nitrogen-incorporated tetrahedral amorphous (ta-C:N) carbon, were compared using flow injection analysis with amperometric detection. These electrodes offer potential improvements in the detection figures of merit as compared to commonly used carbon electrodes. In this work, the background current, noise, and stabilization time were evaluated as a function of the detection from 0.1 to 1.3 V vs. Ag/AgCl. The detection figures of merit for the peptide, alpha-melanocyte stimulating hormone, were also evaluated in 0.1 M phosphate buffer (pH 7.2) for the two electrodes. These figures of merit included the response reproducibility, sensitivity, linear dynamic range, and limit of detection. Data reveal that the ta-C:N electrode exhibits lower background current and noise at all potentials from 0.5 to 1.2 V vs. Ag/AgCl and a significantly lower response variability in repeat injection assays than the BDD electrode. Replicate response curves (n = 3) show equivalent limits of detection, linear dynamic ranges, and sensitivity, and reveals similar r2 values. These results indicate that both ta-C:N and BDD electrodes effectively detect α-MSH, and the ta- C:N electrode may be preferred due to its comparative inexpensiveness, versatility, and lower variability.

Comments

Additional team members: Romana Jarosova

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Apr 28th, 8:30 AM Apr 28th, 9:45 AM

Session 1I: Electrochemical Detection of Alpha-Melanocyte Stimulating Hormone Using Flow Injection Analysis with Amperometric Detection

Room B101

The detection of pharmaceutical compounds, biomolecules, and chemicals plays a critical role in medical care, drug development, and environmental testing. In this study, the analytical performances of two novel materials, boron-doped nanocrystalline diamond (BDD) and nitrogen-incorporated tetrahedral amorphous (ta-C:N) carbon, were compared using flow injection analysis with amperometric detection. These electrodes offer potential improvements in the detection figures of merit as compared to commonly used carbon electrodes. In this work, the background current, noise, and stabilization time were evaluated as a function of the detection from 0.1 to 1.3 V vs. Ag/AgCl. The detection figures of merit for the peptide, alpha-melanocyte stimulating hormone, were also evaluated in 0.1 M phosphate buffer (pH 7.2) for the two electrodes. These figures of merit included the response reproducibility, sensitivity, linear dynamic range, and limit of detection. Data reveal that the ta-C:N electrode exhibits lower background current and noise at all potentials from 0.5 to 1.2 V vs. Ag/AgCl and a significantly lower response variability in repeat injection assays than the BDD electrode. Replicate response curves (n = 3) show equivalent limits of detection, linear dynamic ranges, and sensitivity, and reveals similar r2 values. These results indicate that both ta-C:N and BDD electrodes effectively detect α-MSH, and the ta- C:N electrode may be preferred due to its comparative inexpensiveness, versatility, and lower variability.