Event Title

Session 1K: Suppressor Identification of Disease Resistant Mutant acd6-1

Session Number

Session 1K: 4th Presentation

Advisor(s)

Shang-Chuan Jiang, University of Chicago

Location

Room A119

Start Date

28-4-2017 8:30 AM

End Date

28-4-2002 9:45 AM

Abstract

In order to study the plant defense signaling pathways, mutants of Arabidopsis thaliana were examined for phenotype and levels of salicylic acid (SA) in comparison to gain-of-function mutant acd6-1, which exhibits high disease resistance, dwarfism, and high SA levels. For T-series transformed suppressor mutants, diameter and height were measured in centimeters. Gene expression was also predicted using bioinformatics. T-105 and T-122 genes are expressed in locations similar to acd6-1, and they may be linked to defense regulation. In addition, these mutants suppressed the a6 phenotype the most. A set of a6 double mutants were tested for SA levels using HPLC. For a6 double mutants, all of the double mutants suppressed the high levels of SA found in a6, both when free SA and total SA is calculated. In the future, further testing may be conducted to affirm the candidate suppressors of a6, which display traits relating to regulation of the defense system. Once identified, scientists may overexpress or cut out the specific gene of the mutation depending on whether it upregulates or downregulates the plant defense system, leading to more disease resistant crops, maximizing crop output.

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Apr 28th, 8:30 AM Apr 28th, 9:45 AM

Session 1K: Suppressor Identification of Disease Resistant Mutant acd6-1

Room A119

In order to study the plant defense signaling pathways, mutants of Arabidopsis thaliana were examined for phenotype and levels of salicylic acid (SA) in comparison to gain-of-function mutant acd6-1, which exhibits high disease resistance, dwarfism, and high SA levels. For T-series transformed suppressor mutants, diameter and height were measured in centimeters. Gene expression was also predicted using bioinformatics. T-105 and T-122 genes are expressed in locations similar to acd6-1, and they may be linked to defense regulation. In addition, these mutants suppressed the a6 phenotype the most. A set of a6 double mutants were tested for SA levels using HPLC. For a6 double mutants, all of the double mutants suppressed the high levels of SA found in a6, both when free SA and total SA is calculated. In the future, further testing may be conducted to affirm the candidate suppressors of a6, which display traits relating to regulation of the defense system. Once identified, scientists may overexpress or cut out the specific gene of the mutation depending on whether it upregulates or downregulates the plant defense system, leading to more disease resistant crops, maximizing crop output.