Document Type

Conference Paper/Presentation

Publication Date

2-2012

Advisor(s)

Don Dosch; Illinois Mathematics and Science Academy

Disciplines

Cells | Diseases | Medical Cell Biology | Oncology

Abstract

The ecotropic virus integration site-1 (EVI1) gene is a transcriptional repressor implicated in the control of cell proliferation and frequently over-expressed in cancerous cells. I investigated the expression of this gene across seven cancer cell lines of varying morphologies. The tested lines included leukemia lines Kasumi-3, U937, MOLT-4, and CEM, breast cancer line MCF7, colorectal cancer line HT-29, and glioblastoma line M059K. Kasumi-3 and HT-29 are documented to have high EVI1 expression. Protein concentrations were normalized with respect to actin using SDS-PAGE and Western blotting. Western blots for EVI1 showed expression of an unidentified protein with a molecular weight of 50-53kD in all lines except for Kasumi-3, which had no detectable protein expression. The intensities of these bands were measured and normalized with scaling factors determined from the Western blot for actin. The expression of EVI1 may be below the detection threshold of this blotting system, making visualization of the protein difficult.

Comments

Presented at the American Junior Academy of Sciences conference at the American Association for the Advancement of Science annual conference, February 15-19, 2012 in Vancouver, British Columbia, Canada

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