Apoptotic Gene Response to Nitric Oxide Exposure in Human Carcinoma (A549) Cells
Advisor(s)
James Radosevich, Oral Medicine and Diagnostic Sciences, UIC College of Dentistry
Location
Room A121
Start Date
26-4-2019 10:45 AM
End Date
26-4-2019 11:00 AM
Abstract
Studies have shown that high Nitric Oxide expression in human tumors predicts a poor outcome. In high Nitric Oxide (HNO) adapted cells, signal transduction in response to DNA damage is altered. The objective of this study is to determine what genes are associated with response to DNA damage to nitric oxide exposure. mRNA was isolated from A549 (parent) and A549-HNO cells. The mRNA was converted to cDNA and used to probe a microanalysis array. Genes related to DNA damage with p values of < 0.05, were excluded from analysis. The genes were analyzed for their relationship to each other and other molecular mechanisms, using the GoCode with the Gene Mania function, and Cytoscape. Six genes were identified (ABL1, BID, CASP9, CHEK2, GADD45A, GRB2) that were associated with Nitric oxide expression in relationship to DNA damage; all are activated in response to extra- or intra-cellular stimuli, and then proceed to induce apoptosis. ABL1 and GADD45A were found to be upregulated while the other 4 were found to be down regulated. By studying the genes related to DNA damage upon Nitric Oxide exposure, one can map the molecular phenotype of aggressive tumors, and contribute to developing better therapeutic strategies for cancer treatment.
Apoptotic Gene Response to Nitric Oxide Exposure in Human Carcinoma (A549) Cells
Room A121
Studies have shown that high Nitric Oxide expression in human tumors predicts a poor outcome. In high Nitric Oxide (HNO) adapted cells, signal transduction in response to DNA damage is altered. The objective of this study is to determine what genes are associated with response to DNA damage to nitric oxide exposure. mRNA was isolated from A549 (parent) and A549-HNO cells. The mRNA was converted to cDNA and used to probe a microanalysis array. Genes related to DNA damage with p values of < 0.05, were excluded from analysis. The genes were analyzed for their relationship to each other and other molecular mechanisms, using the GoCode with the Gene Mania function, and Cytoscape. Six genes were identified (ABL1, BID, CASP9, CHEK2, GADD45A, GRB2) that were associated with Nitric oxide expression in relationship to DNA damage; all are activated in response to extra- or intra-cellular stimuli, and then proceed to induce apoptosis. ABL1 and GADD45A were found to be upregulated while the other 4 were found to be down regulated. By studying the genes related to DNA damage upon Nitric Oxide exposure, one can map the molecular phenotype of aggressive tumors, and contribute to developing better therapeutic strategies for cancer treatment.