Event Title

Vitexin enhances BMP9-induced osteogenic differentiation in mesenchymal stem cells (MSCs)

Session Number

Project ID: MEDH 19

Advisor(s)

Dr. Tong-Chuan He; University of Chicago

Dr. Meng Zhang; University of Chicago

Discipline

Medical and Health Sciences

Start Date

22-4-2020 9:45 AM

End Date

22-4-2020 10:00 AM

Workshop Synopsis

Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the potential to differentiate into a variety of cell types, including osteoblasts (bone cells). MSCs are thus applicable in bone regeneration, which can be used to treat bone disorders and injuries and potentially even bone cancer. To better understand the process of MSC differentiation, we investigated the effect of vitexin on the osteogenesis induced by bone morphogenetic protein-9 (BMP-9) in MSCs. We detected osteogenic differentiation by studying Alkaline Phosphatase (ALP) activity and the Alizarin Red S staining of mineralized matrices when iMAD cells were treated with BMP9 and/or Vitexin. Then, the gene expression of the osteogenic markers was detected by quantitative reverse transcription polymerase chain reaction (qPCR). We found that BMP9 and Vitexin increased the activity of early osteogenic differentiation marker ALP. The activities are more pronounced when treated by both BMP9 and Vitexin than when treated by BMP9 or Vitexin alone. These results indicate that Vitexin potentiates the osteogenesis induced by BMP9 in MSCs and is, therefore, a promising method in bone tissue engineering.

Share

COinS
 
Apr 22nd, 9:45 AM Apr 22nd, 10:00 AM

Vitexin enhances BMP9-induced osteogenic differentiation in mesenchymal stem cells (MSCs)