Effect of Knockdown Treatments on Keratinocyte Differentiation
Advisor(s)
Dr. Sui Huang; Northwestern University, Feinberg School of Medicine
Discipline
Biology
Start Date
21-4-2021 9:30 AM
End Date
21-4-2021 9:45 AM
Abstract
Keratinocytes are structures in the human epidermis that highlight the differences between the basal and surface layers of the skin. In a small area, all stages of cell differentiation can be observed in a single keratinocyte sample. The treatments used were siControl Dharamcon, siControl Nitrogen, siNPM1, siRPA194, and siUTP4. For this reason, keratinocytes were used to analyze the changes in cell differentiation between various knockdown treatments. Immunofluorescence was used to obtain images of keratinocytes. For each image, data was collected both on nucleoli (TxRed channel) and centromeres (FITC channel) throughout different stages of differentiation. Each nucleus (DAPI channel), was categorized into one of four layers, with layer one being the basal layer and layer four being the surface layer. The data was then analyzed to determine the effect of treatments on differentiation as well as the role of centromeres and nucleoli in the process. The data suggested that siRPA194 greatly increased the rate of differentiation. (Results of other treatments are still being collected.)
Effect of Knockdown Treatments on Keratinocyte Differentiation
Keratinocytes are structures in the human epidermis that highlight the differences between the basal and surface layers of the skin. In a small area, all stages of cell differentiation can be observed in a single keratinocyte sample. The treatments used were siControl Dharamcon, siControl Nitrogen, siNPM1, siRPA194, and siUTP4. For this reason, keratinocytes were used to analyze the changes in cell differentiation between various knockdown treatments. Immunofluorescence was used to obtain images of keratinocytes. For each image, data was collected both on nucleoli (TxRed channel) and centromeres (FITC channel) throughout different stages of differentiation. Each nucleus (DAPI channel), was categorized into one of four layers, with layer one being the basal layer and layer four being the surface layer. The data was then analyzed to determine the effect of treatments on differentiation as well as the role of centromeres and nucleoli in the process. The data suggested that siRPA194 greatly increased the rate of differentiation. (Results of other treatments are still being collected.)