Event Title

Exploring Ancestral Sequences

Session Number

Project ID: BIO 13

Advisor(s)

Dr. Keith Gagnon; Southern Illinois University, Carbondale

Discipline

Biology

Start Date

20-4-2022 10:25 AM

End Date

20-4-2022 10:40 AM

Abstract

Ancestral sequence reconstruction, commonly abbreviated as ASR, is a method used to estimate the sequence and properties of an extinct organism’s genes. Exploring ancestral sequences has shown the possibility of resurrecting ancient genes for use in current research. Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPR, is used in prokaryotes as their antiviral system. Cas9, an enzyme associated with CRISPR, can be used to cut DNA at specific locations. This has made CRISPR Cas9 a topic of interest as a tool for genetic editing, and by extension its evolutionary history is important to study. Therefore, ancestral sequence reconstruction can be used in order to understand CRISPR Cas9 and its history. The sequences discovered can provide information on its evolution and development as a biotech tool, which will be valuable insight in the genetic engineering field. In this project, I investigate various strains of CRISPR Cas9 and other relating sequences using the maximum likelihood method of ancestral sequence reconstruction to find their closest relative ancestors. This can provide insight into the evolution history of different Cas9 strains, and eventually their use at a larger scale.

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Apr 20th, 10:25 AM Apr 20th, 10:40 AM

Exploring Ancestral Sequences

Ancestral sequence reconstruction, commonly abbreviated as ASR, is a method used to estimate the sequence and properties of an extinct organism’s genes. Exploring ancestral sequences has shown the possibility of resurrecting ancient genes for use in current research. Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPR, is used in prokaryotes as their antiviral system. Cas9, an enzyme associated with CRISPR, can be used to cut DNA at specific locations. This has made CRISPR Cas9 a topic of interest as a tool for genetic editing, and by extension its evolutionary history is important to study. Therefore, ancestral sequence reconstruction can be used in order to understand CRISPR Cas9 and its history. The sequences discovered can provide information on its evolution and development as a biotech tool, which will be valuable insight in the genetic engineering field. In this project, I investigate various strains of CRISPR Cas9 and other relating sequences using the maximum likelihood method of ancestral sequence reconstruction to find their closest relative ancestors. This can provide insight into the evolution history of different Cas9 strains, and eventually their use at a larger scale.