Event Title

The Effects and Role of the Natural Compounds Curcumin and Diallyl Trisulfide on Glioblastoma Cell Lines

Session Number

Q08

Advisor(s)

Don Dosch, Illinois Mathematics and Science Academy

Location

A-133

Start Date

28-4-2016 8:25 AM

End Date

28-4-2016 8:50 AM

Disciplines

Neuroscience and Neurobiology

Abstract

Glioblastoma, one of the most lethal forms of astrocytoma, has a median survival rate of fifteen months post diagnosis. Due to its multiple pathways that allow this cancer to quickly grow, divide, and invade the brain, glioblastoma has a high recurrence rate despite aggressive treatments. Our primary goal for this study is to determine the effect on cell viability of different compounds. Recently, natural substances have been used to impact cell growth of cancer cell lines. Throughout our experiment, we have maintained A172, which is a glioblastoma cell line. Using curcumin and diallyl trisulfide (DATS), compounds found potent against glioblastoma and naturally occurring in foods, we started treating these cells to induce apoptosis. Using the microscope to view the effects curcumin had on the cells, it was concluded that high amounts of curcumin manage to decrease culture growth. After treatment with DATS and curcumin, we determined the viability using an MTT Assay which measures the cellular metabolic activity.

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Apr 28th, 8:25 AM Apr 28th, 8:50 AM

The Effects and Role of the Natural Compounds Curcumin and Diallyl Trisulfide on Glioblastoma Cell Lines

A-133

Glioblastoma, one of the most lethal forms of astrocytoma, has a median survival rate of fifteen months post diagnosis. Due to its multiple pathways that allow this cancer to quickly grow, divide, and invade the brain, glioblastoma has a high recurrence rate despite aggressive treatments. Our primary goal for this study is to determine the effect on cell viability of different compounds. Recently, natural substances have been used to impact cell growth of cancer cell lines. Throughout our experiment, we have maintained A172, which is a glioblastoma cell line. Using curcumin and diallyl trisulfide (DATS), compounds found potent against glioblastoma and naturally occurring in foods, we started treating these cells to induce apoptosis. Using the microscope to view the effects curcumin had on the cells, it was concluded that high amounts of curcumin manage to decrease culture growth. After treatment with DATS and curcumin, we determined the viability using an MTT Assay which measures the cellular metabolic activity.