The Effects of Different Compounds on PLCβ-1 Activity in HL-1 Cells
Session Number
C33
Advisor(s)
Gary Aistrup, Northwestern University William Marszalec, Northwestern University J. Andrew Wasserstrom, Northwestern University
Location
B-110
Start Date
28-4-2016 8:25 AM
End Date
28-4-2016 8:50 AM
Abstract
In hearts, the T-tubules allow calcium ions to enter the organ, which will then initiate a pathway that allows the heart to contract. Studies have shown that over time, T-tubules will lose their structure, leading to less efficient contraction and eventual heart failure. One protein that causes this is phospholipase Cβ-1 (PLCβ-1), which, when activated, cleaves and deactivates phosphatidylinositol 4,5-bisphosphate (PIP2), a protein that supports the stability of T-tubules. Eighteen different chemical compounds were previously determined to be able to theoretically prevent PLCβ-1 from binding to its receptor, protecting T-tubules from remodeling. Our investigation treated HL-1 cells, a type of tumor cell isolated from mouse hearts, with the compounds in order to see which ones were able to lower the activity of PLCβ-1.
The Effects of Different Compounds on PLCβ-1 Activity in HL-1 Cells
B-110
In hearts, the T-tubules allow calcium ions to enter the organ, which will then initiate a pathway that allows the heart to contract. Studies have shown that over time, T-tubules will lose their structure, leading to less efficient contraction and eventual heart failure. One protein that causes this is phospholipase Cβ-1 (PLCβ-1), which, when activated, cleaves and deactivates phosphatidylinositol 4,5-bisphosphate (PIP2), a protein that supports the stability of T-tubules. Eighteen different chemical compounds were previously determined to be able to theoretically prevent PLCβ-1 from binding to its receptor, protecting T-tubules from remodeling. Our investigation treated HL-1 cells, a type of tumor cell isolated from mouse hearts, with the compounds in order to see which ones were able to lower the activity of PLCβ-1.