Event Title

The Analysis of Parkinson's Disease in Mice as Seen Through Astrocyte Morphology

Session Number

Q18

Advisor(s)

Savio Chan, Northwestern University

Location

A-133

Start Date

28-4-2016 1:10 PM

End Date

28-4-2016 1:35 PM

Disciplines

Neuroscience and Neurobiology

Abstract

Parkinson’s Disease (PD) is a progressive nervous system disorder that causes the dysfunction and the gradual death of vital nerve cells. The pathology in the nerve cells can cause various motor dysfunctions, such as tremors, rigidity, postural instability etc. Glial cells, like astrocytes, are the most abundant cell type in the brain and ensure the proper function of surrounding neurons and helps sustain the communication in neuronal circuits. In this investigation, mice that are injected with agents that induce PD-like pathology and behavior are used to study astrocyte morphology. In ex vivo slices, astrocytes are injected with a fluorescent dye to fill the entire cell body and their processes. Confocal microscopy is then used to obtain stacked images of the astrocyte in ImageJ. Using a Sholl Analysis, data of the cell body size and total length and branch size of astrocyte projections can be obtained. This data will demonstrate the complexity of the astrocytes’ function and determine the differences in astrocyte morphology in cell from control and PD mice.


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Apr 28th, 1:10 PM Apr 28th, 1:35 PM

The Analysis of Parkinson's Disease in Mice as Seen Through Astrocyte Morphology

A-133

Parkinson’s Disease (PD) is a progressive nervous system disorder that causes the dysfunction and the gradual death of vital nerve cells. The pathology in the nerve cells can cause various motor dysfunctions, such as tremors, rigidity, postural instability etc. Glial cells, like astrocytes, are the most abundant cell type in the brain and ensure the proper function of surrounding neurons and helps sustain the communication in neuronal circuits. In this investigation, mice that are injected with agents that induce PD-like pathology and behavior are used to study astrocyte morphology. In ex vivo slices, astrocytes are injected with a fluorescent dye to fill the entire cell body and their processes. Confocal microscopy is then used to obtain stacked images of the astrocyte in ImageJ. Using a Sholl Analysis, data of the cell body size and total length and branch size of astrocyte projections can be obtained. This data will demonstrate the complexity of the astrocytes’ function and determine the differences in astrocyte morphology in cell from control and PD mice.