Session D-6: Green Fluorescent Protein
Session Number
D-6
Location
A206
Grade Level
Grade Level: 9-12
Start Date
25-2-2011 1:00 PM
End Date
24-2-2011 2:00 PM
Description
GFP purification requires four major steps: inoculation of colonies and growing the cell culture; lysis of cell membrane to extract the protein; purification of protein using hydrophobic column; and use of SDS electrophoresis gel to check for purity. Stations will be set up for you to experience these steps. You can use this experience to adapt this lab in your own classroom or teach students about biotechnology.
Session D-6: Green Fluorescent Protein
A206
GFP purification requires four major steps: inoculation of colonies and growing the cell culture; lysis of cell membrane to extract the protein; purification of protein using hydrophobic column; and use of SDS electrophoresis gel to check for purity. Stations will be set up for you to experience these steps. You can use this experience to adapt this lab in your own classroom or teach students about biotechnology.