Visualizing Bacterial Alarmone Induction and Antibiotic Survival in Bacillius subtilis.
Session Number
C34
Advisor(s)
Kachun Danny Fung, University of Wisconsin at Madison Jue (Jade) Wang, University of Wisconsin at Madison
Location
B-116
Start Date
28-4-2016 9:50 AM
End Date
28-4-2016 10:15 AM
Abstract
Guanosine penta/tetraphosphate ((p)ppGpp) plays an influential role in the stress response exhibited by bacteria cells. Not only does it prevent the production of GTP, but it also helps to prevent cellular death. ppGpp induction is found under the presence of amino acid starvation and also inhibits GTP biosynthesis enzymes. Furthermore, there is a direct correlation between cell death and the dysregulation of GTP caused by a lack of ppGpp. The work of this study aimed to identify the effects of ppGpp induction on Bacillus subtilis under the stress of antibiotics such as vancomycin, trimethoprim, and carbenicillin. Killing curves were run to identify the effects of vancomycin on ppGpp induction. Single cell time-lapse microscopy analysis and mCherry reporters were used to identify cells that were spontaneously induced by ppGpp. Transposon sequencing was used to identify genes that were necessary in the induction of ppGpp. The results of this study found that spontaneous induction of ppGpp is common and that it does indeed hinder cell processes, including cell growth. In addition, the ppGpp is useful in preventing cell death and is a significant portion in the survival of B. subtilis through external stressors.
Visualizing Bacterial Alarmone Induction and Antibiotic Survival in Bacillius subtilis.
B-116
Guanosine penta/tetraphosphate ((p)ppGpp) plays an influential role in the stress response exhibited by bacteria cells. Not only does it prevent the production of GTP, but it also helps to prevent cellular death. ppGpp induction is found under the presence of amino acid starvation and also inhibits GTP biosynthesis enzymes. Furthermore, there is a direct correlation between cell death and the dysregulation of GTP caused by a lack of ppGpp. The work of this study aimed to identify the effects of ppGpp induction on Bacillus subtilis under the stress of antibiotics such as vancomycin, trimethoprim, and carbenicillin. Killing curves were run to identify the effects of vancomycin on ppGpp induction. Single cell time-lapse microscopy analysis and mCherry reporters were used to identify cells that were spontaneously induced by ppGpp. Transposon sequencing was used to identify genes that were necessary in the induction of ppGpp. The results of this study found that spontaneous induction of ppGpp is common and that it does indeed hinder cell processes, including cell growth. In addition, the ppGpp is useful in preventing cell death and is a significant portion in the survival of B. subtilis through external stressors.