Effect of Hormones on Breast Cancer Cell Line Enhancers
Session Number
C27
Advisor(s)
Michael Bolt, University of Chicago Heather Scott, University of Chicago Kevin White, University of Chicago
Location
B-131 Grainger
Start Date
28-4-2016 1:35 PM
End Date
28-4-2016 2:00 PM
Abstract
Breast cancer pervades society, so its cell lines are studied to find therapies. Hormones and enhancer regions play a central role in messenger ribonucleic acid (mRNA) levels of genes, so observing them can help develop new therapies. We use a luminal A breast cancer cell line model, MCF-7, to determine the effect of different hormones on gene expression. Hormone effect on gene expression within cell lines needed to be determined by Quantitative Polymerase Chain Reaction (qPCR), a process that determines the expression levels of ribonucleic acids of specific gene targets. Preliminary results suggest each MCF- 7 ligand used (E2, Dexamethasone, GW0742, AM580, T3, and Rosiglitazone) showed hormone specific effects in gene expression. T3 and ROSI both primarily up-regulate their targets, while the remaining hormones are more of a mix of up and down regulated genes. However, future studies will have to confirm this using mRNA sequencing to determine the genome-wide transcription effects of these hormone. CapStarr-seq (self-transcribing active regulatory region sequencing) will be used to determine enhancers involved. By studying the interactions of hormones on MCF-7, we hope to further understand cell line interactions in breast cancer for greater therapeutic options in patients and understand this form of breast cancer better.
Effect of Hormones on Breast Cancer Cell Line Enhancers
B-131 Grainger
Breast cancer pervades society, so its cell lines are studied to find therapies. Hormones and enhancer regions play a central role in messenger ribonucleic acid (mRNA) levels of genes, so observing them can help develop new therapies. We use a luminal A breast cancer cell line model, MCF-7, to determine the effect of different hormones on gene expression. Hormone effect on gene expression within cell lines needed to be determined by Quantitative Polymerase Chain Reaction (qPCR), a process that determines the expression levels of ribonucleic acids of specific gene targets. Preliminary results suggest each MCF- 7 ligand used (E2, Dexamethasone, GW0742, AM580, T3, and Rosiglitazone) showed hormone specific effects in gene expression. T3 and ROSI both primarily up-regulate their targets, while the remaining hormones are more of a mix of up and down regulated genes. However, future studies will have to confirm this using mRNA sequencing to determine the genome-wide transcription effects of these hormone. CapStarr-seq (self-transcribing active regulatory region sequencing) will be used to determine enhancers involved. By studying the interactions of hormones on MCF-7, we hope to further understand cell line interactions in breast cancer for greater therapeutic options in patients and understand this form of breast cancer better.