Session 2D: The Function of gD and gD-Fc in Relation to Herpes Simplex Virus Entry and Fusion
Session Number
Session 2D: 1st Presentation
Advisor(s)
Deepak Shukla, University of Illinois at Chicago
Location
Room A155
Start Date
28-4-2017 10:00 AM
End Date
28-4-2017 11:15 AM
Abstract
The Herpes Simplex Virus (HSV), known as herpes, is a part of the herpesviridae family. There are glycoproteins on the surface of HSV. These glycoproteins are activated with their receptors on the cell surface causing the viral particles to sure into the cells and get endocytosed by the host cells. This investigation was conducted to figure out how exactly gD, a HSV glycoprotein, worked in human corneal epithelial (HCE) cells. Using morphology imaging and Western blots, our results show that gD alone was not able to travel outside the cell, but with the Fc attached to the gD, which yielded gD-Fc, the gD was able to travel to the outside of the cell. This suggests that HSV may have a mechanism similar to Fc in the fusion process to help the virus spread to other cells. This also may suggest that gD plays a role in the fusion process more than the entry.
Session 2D: The Function of gD and gD-Fc in Relation to Herpes Simplex Virus Entry and Fusion
Room A155
The Herpes Simplex Virus (HSV), known as herpes, is a part of the herpesviridae family. There are glycoproteins on the surface of HSV. These glycoproteins are activated with their receptors on the cell surface causing the viral particles to sure into the cells and get endocytosed by the host cells. This investigation was conducted to figure out how exactly gD, a HSV glycoprotein, worked in human corneal epithelial (HCE) cells. Using morphology imaging and Western blots, our results show that gD alone was not able to travel outside the cell, but with the Fc attached to the gD, which yielded gD-Fc, the gD was able to travel to the outside of the cell. This suggests that HSV may have a mechanism similar to Fc in the fusion process to help the virus spread to other cells. This also may suggest that gD plays a role in the fusion process more than the entry.
Comments
Additional team members: Dinesh Jaishankar