Session 2D: Molecular Cloning and Characterization of Short RNA Sequences That May Interrupt Osteogenic Differentiation of Mesenchymal Stem Cells

Session Number

Session 2D: 4th Presentation

Advisor(s)

Tong-Chuan He, University of Chicago

Location

Room A155

Start Date

28-4-2017 10:00 AM

End Date

28-4-2017 11:15 AM

Abstract

Osteosarcoma can be regarded as a differentiation disease that is caused by genetic and epigenetic disruptions of osteoblast terminal differentiation. Using an innovative approach of genome-wide functional screening, we have recently uncovered that forced expression of certain short RNA (19-base) may disrupt BMP9-induced bone formation from MSCs. These short RNA are designated as Disruptors of Osteogenesis induced by BMP9 (or DONs). This study determines that the candidate DONs can effectively disrupt osteogenic differentiation and bone formation from MSCs, rendering MSCs bone tumor-like phenotype in a mouse model.

Comments

Additional team members: Dr. Junyi Liao

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Apr 28th, 10:00 AM Apr 28th, 11:15 AM

Session 2D: Molecular Cloning and Characterization of Short RNA Sequences That May Interrupt Osteogenic Differentiation of Mesenchymal Stem Cells

Room A155

Osteosarcoma can be regarded as a differentiation disease that is caused by genetic and epigenetic disruptions of osteoblast terminal differentiation. Using an innovative approach of genome-wide functional screening, we have recently uncovered that forced expression of certain short RNA (19-base) may disrupt BMP9-induced bone formation from MSCs. These short RNA are designated as Disruptors of Osteogenesis induced by BMP9 (or DONs). This study determines that the candidate DONs can effectively disrupt osteogenic differentiation and bone formation from MSCs, rendering MSCs bone tumor-like phenotype in a mouse model.