Session 2G: The Effect of Increased NaCl Concentration on Macrophage Inflammation
Session Number
Session 2G:3rd Presentation
Advisor(s)
Seok Jong Hong, Northwestern University
Location
Room A117
Start Date
28-4-2017 10:00 AM
End Date
28-4-2017 11:15 AM
Abstract
High salt has shown to be a part of the human lifestyle for ages, but has had adverse effects on health. High salt’s influence not only encompasses nutrition but also involves wound healing. During the process of a wound, an increase in Na ions is known to lead towards an inflammatory reaction. We demonstrate that macrophages, as one of many cells exposed to the wound healing process, does not show an inflammatory response when exposed to high salt. The macrophages were isolated from the femur and tibia of a rat’s hind limbs. The differentiated macrophages were stimulated with a 10% and 20% increase in media NaCl. The RNA was isolated from the macrophages and was analyzed through qPCR. The macrophages did not demonstrate an inflammatory response. The result helped demonstrate that macrophages were not another cell type that played a role in the inflammation due to high salt. This clarification further demonstrated that macrophages lacked the NaX channel that is seen in cells which demonstrate inflammatory response to high salt.
Session 2G: The Effect of Increased NaCl Concentration on Macrophage Inflammation
Room A117
High salt has shown to be a part of the human lifestyle for ages, but has had adverse effects on health. High salt’s influence not only encompasses nutrition but also involves wound healing. During the process of a wound, an increase in Na ions is known to lead towards an inflammatory reaction. We demonstrate that macrophages, as one of many cells exposed to the wound healing process, does not show an inflammatory response when exposed to high salt. The macrophages were isolated from the femur and tibia of a rat’s hind limbs. The differentiated macrophages were stimulated with a 10% and 20% increase in media NaCl. The RNA was isolated from the macrophages and was analyzed through qPCR. The macrophages did not demonstrate an inflammatory response. The result helped demonstrate that macrophages were not another cell type that played a role in the inflammation due to high salt. This clarification further demonstrated that macrophages lacked the NaX channel that is seen in cells which demonstrate inflammatory response to high salt.
Comments
Additional team members: Elena Bogdanovic