Session 2H: The DNA Cleaving Properties of Enediyne Coated Carbon Paper Treated in UV Light and Heat
Session Number
Session 2H: 2nd Presentation
Advisor(s)
Dipanjan Pan, University of Illinois at Urbana-Champaign
Location
Room B101
Start Date
28-4-2017 10:00 AM
End Date
28-4-2017 11:15 AM
Abstract
The wastewater from hospitals contains many hazardous materials such as antibiotic, genetic material, and bacteria. It is essential to treat this wastewater fully by removing all of this hazardous material before it enters aquatic environments or is in contact with humans. Enediyne is a class of small molecule that can cleave DNA in tumors by formation of biradical. We tested enediyne in a hospital wastewater application to optimize the cleaving of the genetic material found in hospital wastewater. We placed enediyne on carbon coated paper and then tested the success of DNA cleavage under both Heat and UV light exposure through the gel electrophoresis process. The process was adjusted until optimal DNA cleaving had occurred. The results showed that while both Heat and UV light activated enediyne resulting in DNA cleavage, UV Light was the more effective activator of enediyne and thus attained more success in DNA cleavage. Therefore, enediyne can be used as a treatment option to destroy the hazardous genetic material in hospital waste waters.
Session 2H: The DNA Cleaving Properties of Enediyne Coated Carbon Paper Treated in UV Light and Heat
Room B101
The wastewater from hospitals contains many hazardous materials such as antibiotic, genetic material, and bacteria. It is essential to treat this wastewater fully by removing all of this hazardous material before it enters aquatic environments or is in contact with humans. Enediyne is a class of small molecule that can cleave DNA in tumors by formation of biradical. We tested enediyne in a hospital wastewater application to optimize the cleaving of the genetic material found in hospital wastewater. We placed enediyne on carbon coated paper and then tested the success of DNA cleavage under both Heat and UV light exposure through the gel electrophoresis process. The process was adjusted until optimal DNA cleaving had occurred. The results showed that while both Heat and UV light activated enediyne resulting in DNA cleavage, UV Light was the more effective activator of enediyne and thus attained more success in DNA cleavage. Therefore, enediyne can be used as a treatment option to destroy the hazardous genetic material in hospital waste waters.