Session 3H: Defining the differences in EphA2 signaling networks in early versus late stage differentiation
Session Number
Session 3H: 1st Presentation
Advisor(s)
Drs. Bethany E. Perez-White and Nihal Kaplan, and Rosa Ventrella, Northwestern University
Location
Room A117
Start Date
26-4-2018 12:40 PM
End Date
26-4-2018 1:25 PM
Abstract
Skin is a stratified epithelium consisting of several layers of cells in various stages of differentiation. EphA2 receptor tyrosine kinase, a signaling molecule known to facilitate cellular communication and prompt keratinocyte differentiation, has a distinct expression pattern in these different cell layers. Our data show that EphA2 protein has a diffuse, pan-cellular expression pattern in the proliferative layer of epidermis. In contrast, EphA2 localizes at the cellular membrane in the differentiated cells of the upper layers. These observations led us to hypothesize that EphA2 participates in unique signaling pathways in early- versus late-stage epidermal differentiation. We performed biotin-identification proteomics (BioID) to identify the EphA2 interactions at different stages of differentiation. The results from this screen revealed 372 total EphA2-interacting proteins in early stages of differentiation and 192 proteins at later differentiation, of these, 86 were found to be common between both time points. Gene ontology term (GO term) analysis found different biological processes associated with EphA2 at early versus late differentiation. In early differentiation, vesicle mediated transport was the primary biological process. Whereas in late differentiation, regulation of cytoskeleton organization was the top GO term. Based on these results we will further probe the distinct roles EphA2 has during early or late keratinocyte differentiation.
Session 3H: Defining the differences in EphA2 signaling networks in early versus late stage differentiation
Room A117
Skin is a stratified epithelium consisting of several layers of cells in various stages of differentiation. EphA2 receptor tyrosine kinase, a signaling molecule known to facilitate cellular communication and prompt keratinocyte differentiation, has a distinct expression pattern in these different cell layers. Our data show that EphA2 protein has a diffuse, pan-cellular expression pattern in the proliferative layer of epidermis. In contrast, EphA2 localizes at the cellular membrane in the differentiated cells of the upper layers. These observations led us to hypothesize that EphA2 participates in unique signaling pathways in early- versus late-stage epidermal differentiation. We performed biotin-identification proteomics (BioID) to identify the EphA2 interactions at different stages of differentiation. The results from this screen revealed 372 total EphA2-interacting proteins in early stages of differentiation and 192 proteins at later differentiation, of these, 86 were found to be common between both time points. Gene ontology term (GO term) analysis found different biological processes associated with EphA2 at early versus late differentiation. In early differentiation, vesicle mediated transport was the primary biological process. Whereas in late differentiation, regulation of cytoskeleton organization was the top GO term. Based on these results we will further probe the distinct roles EphA2 has during early or late keratinocyte differentiation.