Identifying effects of stress on RNA editing in a rat brain model of PTSD
Advisor(s)
Monsheel Sodhi, Loyola University Chicago
Location
Room B108-1
Start Date
26-4-2019 10:45 AM
End Date
26-4-2019 11:00 AM
Abstract
This project analyzes the effects of environmental stress on levels of RNA editing in an adult rat brain PTSD model to search for implications in mental health. We have previously detected changes in the levels of RNA editing due to prenatal stress, but we have not tested a model of adult stress. We plan to investigate changes in the levels of RNA editing through NGS (next-generation sequencing) of cDNA with the aid of specifically designed primers at RNA editing sites. This project focuses on alterations in levels of RNA editing specifically in the hippocampus of the rat brain model as literature in the field has shown the hippocampus to be heavily involved in the animal stress response. Through the usage of specific RNA sequences that are conserved between rats and humans, this project aims to extrapolate its findings to increase current understanding in the field of the molecular basis of PTSD in humans.
Identifying effects of stress on RNA editing in a rat brain model of PTSD
Room B108-1
This project analyzes the effects of environmental stress on levels of RNA editing in an adult rat brain PTSD model to search for implications in mental health. We have previously detected changes in the levels of RNA editing due to prenatal stress, but we have not tested a model of adult stress. We plan to investigate changes in the levels of RNA editing through NGS (next-generation sequencing) of cDNA with the aid of specifically designed primers at RNA editing sites. This project focuses on alterations in levels of RNA editing specifically in the hippocampus of the rat brain model as literature in the field has shown the hippocampus to be heavily involved in the animal stress response. Through the usage of specific RNA sequences that are conserved between rats and humans, this project aims to extrapolate its findings to increase current understanding in the field of the molecular basis of PTSD in humans.