Honokiol and Cisplatin Impact on Ototoxicity from Chemotherapy
Advisor(s)
Claus-Peter Richter, MD, Phd. Vice Chair for Research, Department of Otolaryngology
Xiaodong Tan, Phd. Research Assistant Professor of Otolaryngology, Feinberg School of Medicine
Location
Room A123-2
Start Date
26-4-2019 10:45 AM
End Date
26-4-2019 11:00 AM
Abstract
In this experiment, ototoxicity from the chemotherapeutic drug cisplatin was studied to reduce or eliminate this common side effect from chemotherapy. Cisplatin causes damage to mitochondria and DNA in ear cells, resulting in severe hearing loss and deafness. Use of the drug Honokiol, a traditional Chinese medicine, is being tested in conjunction with chemotherapy to combat the ototoxicity from cisplatin and prevent hearing loss. In order to determine the effectiveness of this drug, two groups of mice are treated with cisplatin and with cisplatin and Honokiol and their hearing abilities are monitored. After specific intervals of time, the hearing ability of the mice was recorded by monitoring the reactivity in their brains after sounds were played at predetermined frequencies. Overtime, this determines how the hearing loss of the mice has progressed and provides a comparison between hearing loss from cisplatin and hearing loss from cisplatin with Honokiol. In order to confirm these results, x-ray fluorescence is used to study the ions in the cochlea of the individual mice. Our results initial results show a significant difference between treatment of mice with chemotherapy and treatment of mice with chemotherapy and Honokiol, indicated that the Honokiol does prevent ototoxicity.
Honokiol and Cisplatin Impact on Ototoxicity from Chemotherapy
Room A123-2
In this experiment, ototoxicity from the chemotherapeutic drug cisplatin was studied to reduce or eliminate this common side effect from chemotherapy. Cisplatin causes damage to mitochondria and DNA in ear cells, resulting in severe hearing loss and deafness. Use of the drug Honokiol, a traditional Chinese medicine, is being tested in conjunction with chemotherapy to combat the ototoxicity from cisplatin and prevent hearing loss. In order to determine the effectiveness of this drug, two groups of mice are treated with cisplatin and with cisplatin and Honokiol and their hearing abilities are monitored. After specific intervals of time, the hearing ability of the mice was recorded by monitoring the reactivity in their brains after sounds were played at predetermined frequencies. Overtime, this determines how the hearing loss of the mice has progressed and provides a comparison between hearing loss from cisplatin and hearing loss from cisplatin with Honokiol. In order to confirm these results, x-ray fluorescence is used to study the ions in the cochlea of the individual mice. Our results initial results show a significant difference between treatment of mice with chemotherapy and treatment of mice with chemotherapy and Honokiol, indicated that the Honokiol does prevent ototoxicity.