Investigating Differences in Oral Microbiomes in Varying Dietary Choices
Session Number
Project ID: BIO 14
Advisor(s)
Dr. Sowmya Anjur; Illinois Mathematics and Science Academy
Dr. Don Dosch; Illinois Mathematics and Science Academy
Discipline
Biology
Start Date
22-4-2020 8:50 AM
End Date
22-4-2020 9:05 AM
Abstract
With over 700 species of bacteria, the oral microbiome is one of the most diverse environments of the human body, second only to the gut. Due to its importance in human health, the gut microbiome has been more extensively studied than any other human body microbiome, leaving far less research on the other microbiomes. The oral microbiome has been connected to systemic diseases in humans, including diabetes and cardiovascular disease. This study looked to discover differences in the presence of various genera of bacteria within the oral microbiomes, specifically regarding subjects’ diets. Polymerase chain reactions were employed using genera-specific primers to amplify the bacterial DNA within the samples before they were run through gel electrophoresis to determine the specific bacteria present. A limited number of samples were isolated and amplified and I used those samples to develop a procedure for isolating DNA from subjects’ oral cavities and amplifying the bacterial DNA within the samples.
Investigating Differences in Oral Microbiomes in Varying Dietary Choices
With over 700 species of bacteria, the oral microbiome is one of the most diverse environments of the human body, second only to the gut. Due to its importance in human health, the gut microbiome has been more extensively studied than any other human body microbiome, leaving far less research on the other microbiomes. The oral microbiome has been connected to systemic diseases in humans, including diabetes and cardiovascular disease. This study looked to discover differences in the presence of various genera of bacteria within the oral microbiomes, specifically regarding subjects’ diets. Polymerase chain reactions were employed using genera-specific primers to amplify the bacterial DNA within the samples before they were run through gel electrophoresis to determine the specific bacteria present. A limited number of samples were isolated and amplified and I used those samples to develop a procedure for isolating DNA from subjects’ oral cavities and amplifying the bacterial DNA within the samples.