Using MALDI TOF-MS and IDBac pipeline to develop a “smart” Vietnamese microbial library for drug discovery
Session Number
Project ID: BIO 15
Advisor(s)
Linh Nguyen; University of Illinois at Chicago, College of Pharmacy
Brian T. Murphy; University of Illinois at Chicago, College of Pharmacy,
Discipline
Biology
Start Date
22-4-2020 9:10 AM
End Date
22-4-2020 9:25 AM
Abstract
Microorganisms have provided abundant sources of natural products which have been developed as commercial products for human medicine, animal health, and plant crop protection. Small molecules produced by actinomycete bacteria have been essential components of antibacterial drug discovery. However, the discovery of new drug leads is being outpaced by the development of antibiotic resistance to current medicines. We strive to build a smart library of bacteria using a MALDI-TOF MS-based bioinformatics pipeline (IDBac). Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry(MALDI-TOF MS) analyzes complex biological systems such as bacterial cells. This technique detects mainly the most abundant and conserved ribosomal protein fractions of bacteria which can be used for classification of the organisms. IDBac is a data acquisition and bioinformatics technique that utilizes MALDI-TOF MS to analyze protein and specialized metabolite spectra recorded from single bacterial colonies picked from agar plates. IDBac ensures that each strain in our library is taxonomically diverse. The collection of aquatic bacteria can be used to generate natural product antibiotic drug-leads for infectious diseases.
Using MALDI TOF-MS and IDBac pipeline to develop a “smart” Vietnamese microbial library for drug discovery
Microorganisms have provided abundant sources of natural products which have been developed as commercial products for human medicine, animal health, and plant crop protection. Small molecules produced by actinomycete bacteria have been essential components of antibacterial drug discovery. However, the discovery of new drug leads is being outpaced by the development of antibiotic resistance to current medicines. We strive to build a smart library of bacteria using a MALDI-TOF MS-based bioinformatics pipeline (IDBac). Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry(MALDI-TOF MS) analyzes complex biological systems such as bacterial cells. This technique detects mainly the most abundant and conserved ribosomal protein fractions of bacteria which can be used for classification of the organisms. IDBac is a data acquisition and bioinformatics technique that utilizes MALDI-TOF MS to analyze protein and specialized metabolite spectra recorded from single bacterial colonies picked from agar plates. IDBac ensures that each strain in our library is taxonomically diverse. The collection of aquatic bacteria can be used to generate natural product antibiotic drug-leads for infectious diseases.