Using MALDI TOF-MS and IDBac pipeline to develop a “smart” Vietnamese microbial library for drug discovery

Session Number

Project ID: BIO 15

Advisor(s)

Linh Nguyen; University of Illinois at Chicago, College of Pharmacy

Brian T. Murphy; University of Illinois at Chicago, College of Pharmacy,

Discipline

Biology

Start Date

22-4-2020 9:10 AM

End Date

22-4-2020 9:25 AM

Abstract

Microorganisms have provided abundant sources of natural products which have been developed as commercial products for human medicine, animal health, and plant crop protection. Small molecules produced by actinomycete bacteria have been essential components of antibacterial drug discovery. However, the discovery of new drug leads is being outpaced by the development of antibiotic resistance to current medicines. We strive to build a smart library of bacteria using a MALDI-TOF MS-based bioinformatics pipeline (IDBac). Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry(MALDI-TOF MS) analyzes complex biological systems such as bacterial cells. This technique detects mainly the most abundant and conserved ribosomal protein fractions of bacteria which can be used for classification of the organisms. IDBac is a data acquisition and bioinformatics technique that utilizes MALDI-TOF MS to analyze protein and specialized metabolite spectra recorded from single bacterial colonies picked from agar plates. IDBac ensures that each strain in our library is taxonomically diverse. The collection of aquatic bacteria can be used to generate natural product antibiotic drug-leads for infectious diseases.

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Apr 22nd, 9:10 AM Apr 22nd, 9:25 AM

Using MALDI TOF-MS and IDBac pipeline to develop a “smart” Vietnamese microbial library for drug discovery

Microorganisms have provided abundant sources of natural products which have been developed as commercial products for human medicine, animal health, and plant crop protection. Small molecules produced by actinomycete bacteria have been essential components of antibacterial drug discovery. However, the discovery of new drug leads is being outpaced by the development of antibiotic resistance to current medicines. We strive to build a smart library of bacteria using a MALDI-TOF MS-based bioinformatics pipeline (IDBac). Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry(MALDI-TOF MS) analyzes complex biological systems such as bacterial cells. This technique detects mainly the most abundant and conserved ribosomal protein fractions of bacteria which can be used for classification of the organisms. IDBac is a data acquisition and bioinformatics technique that utilizes MALDI-TOF MS to analyze protein and specialized metabolite spectra recorded from single bacterial colonies picked from agar plates. IDBac ensures that each strain in our library is taxonomically diverse. The collection of aquatic bacteria can be used to generate natural product antibiotic drug-leads for infectious diseases.