Investigating Sex-Specific Effects of Gut Microbiome Perturbations on Alzheimer’s Pathology

Session Number

Project ID: MEDH 33

Advisor(s)

Dr. Sangram Sisodia; University of Chicago, Pritzker School of Medicine

Discipline

Medical and Health Sciences

Start Date

22-4-2020 9:45 AM

End Date

22-4-2020 10:00 AM

Abstract

Current evidence of the microbiome-gut-brain axis suggests that gut microbiome plays a key role in regulating microglial maturation and function. An altered microbiome in the gut has been characterized in Alzheimer’s disease (AD). In prior studies, the Sisodia lab has shown that antibiotic cocktail (ABX)-perturbed altered gut microbiome in APPPS1-21 male mice showed reduced brain amyloidosis which were restored after fecal microbiota transplantation (FMT) of age-matched APPPS1-21 mice. This demonstrates a causal relationship between AB plaque deposits and the gut microbiome in AD mouse models. However, the microbiome differences in APPPS1-21 transgenic (Tg) and Wild-Type (Wt) mice and their effects on AB pathology remains unknown. Here I propose FMT transplants of gut microbiome from Tg male mice and Wt male mice into ABX-treated APPS1-21 male mice to investigate any differences in their gut microbiome and the cause of AB pathology.

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Apr 22nd, 9:45 AM Apr 22nd, 10:00 AM

Investigating Sex-Specific Effects of Gut Microbiome Perturbations on Alzheimer’s Pathology

Current evidence of the microbiome-gut-brain axis suggests that gut microbiome plays a key role in regulating microglial maturation and function. An altered microbiome in the gut has been characterized in Alzheimer’s disease (AD). In prior studies, the Sisodia lab has shown that antibiotic cocktail (ABX)-perturbed altered gut microbiome in APPPS1-21 male mice showed reduced brain amyloidosis which were restored after fecal microbiota transplantation (FMT) of age-matched APPPS1-21 mice. This demonstrates a causal relationship between AB plaque deposits and the gut microbiome in AD mouse models. However, the microbiome differences in APPPS1-21 transgenic (Tg) and Wild-Type (Wt) mice and their effects on AB pathology remains unknown. Here I propose FMT transplants of gut microbiome from Tg male mice and Wt male mice into ABX-treated APPS1-21 male mice to investigate any differences in their gut microbiome and the cause of AB pathology.