The Effects of Probiotics through Maternal Administration in the Gut Development of Pups
Session Number
Project ID: BIO 08
Advisor(s)
Dr. Erika Claud; University of Chicago
Discipline
Biology
Start Date
22-4-2020 10:25 AM
End Date
22-4-2020 10:40 AM
Abstract
Probiotics have been found to be useful in reducing necrotizing enterocolitis in preterm infants. However, many times these infants are unable to absorb the probiotics due to the poor ability for some microbes to finish colonizing the gut. Early microbial colonization has shown the potential to improve the immune system in infants and therefore is crucial to the short- and long-term well-being of the infant. A mouse model was used to understand how treating the damps with the probiotics could affect the gut microbiota and the small intestinal development of the pup. Markers such as enteroendocrine cells, goblet cells, and Paneth cells throughout the villi and crypts of the sectioned tissues through typical immunofluorescent and enzymatic stainings were used to describe levels of the immune responses and stages of development in the small intestine. The stainings indicated that the probiotics facilitated the development of these cells and promoted the integrity of the immune system and inflammatory response towards threats. These results indicate that probiotics may be administered through the damp and may be a safer method to reduce necrotizing enterocolitis in preterm infants
The Effects of Probiotics through Maternal Administration in the Gut Development of Pups
Probiotics have been found to be useful in reducing necrotizing enterocolitis in preterm infants. However, many times these infants are unable to absorb the probiotics due to the poor ability for some microbes to finish colonizing the gut. Early microbial colonization has shown the potential to improve the immune system in infants and therefore is crucial to the short- and long-term well-being of the infant. A mouse model was used to understand how treating the damps with the probiotics could affect the gut microbiota and the small intestinal development of the pup. Markers such as enteroendocrine cells, goblet cells, and Paneth cells throughout the villi and crypts of the sectioned tissues through typical immunofluorescent and enzymatic stainings were used to describe levels of the immune responses and stages of development in the small intestine. The stainings indicated that the probiotics facilitated the development of these cells and promoted the integrity of the immune system and inflammatory response towards threats. These results indicate that probiotics may be administered through the damp and may be a safer method to reduce necrotizing enterocolitis in preterm infants