The Influence of Various Drug States on the Responses to External Stimuli through Simulation in a Rodent Model

Session Number

Project ID: BIO 35

Advisor(s)

Dr. Maria Virginia Centeno; Northwestern Feinberg School of Medicine

Dr. A. Vania Apkarian; Northwestern Feinberg School of Medicine

Discipline

Biology

Start Date

22-4-2020 10:25 AM

End Date

22-4-2020 10:40 AM

Abstract

Opioids are consistently prescribed as pain management drugs for patients. However, the physiological impact of opioids on the brain are not very well understood, specifically in determining the neurotransmitters affected by the drugs. Morphine, a narcotic analgesic, is used to treat severe pain by changing how the brain feels and responds to pain but it is unknown exactly how the drug alters the brain. Hence, a nociceptive assay was performed on rodent models in order to characterize the physiological effects of morphine. A group of rats were injected with morphine and saline or a combination of levodopa and carbidopa to determine their basic pain-based reflexes. A traditional two-hour infrared tail flick test was administered to measure the pain reflex time, with a baseline test taken previous to injection. A distinction was also made between male and female rats and this comparative model was tested multiple times.

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Apr 22nd, 10:25 AM Apr 22nd, 10:40 AM

The Influence of Various Drug States on the Responses to External Stimuli through Simulation in a Rodent Model

Opioids are consistently prescribed as pain management drugs for patients. However, the physiological impact of opioids on the brain are not very well understood, specifically in determining the neurotransmitters affected by the drugs. Morphine, a narcotic analgesic, is used to treat severe pain by changing how the brain feels and responds to pain but it is unknown exactly how the drug alters the brain. Hence, a nociceptive assay was performed on rodent models in order to characterize the physiological effects of morphine. A group of rats were injected with morphine and saline or a combination of levodopa and carbidopa to determine their basic pain-based reflexes. A traditional two-hour infrared tail flick test was administered to measure the pain reflex time, with a baseline test taken previous to injection. A distinction was also made between male and female rats and this comparative model was tested multiple times.