In vitro analysis of an ultraporous synthetic scaffold's ability to promote mesenchymal stem cell survival and chondrogenesis
Session Number
Project ID: MEDH 26
Advisor(s)
Dr. Tong-Chuan He, University of Chicago, Pritzker School of Medicine
Dr. Russell Reid, University of Chicago, Pritzker School of Medicine
Dr. Bryce Hendren-Santiago, University of Chicago, Pritzker School of Medicine
Discipline
Medical and Health Sciences
Start Date
20-4-2022 8:50 AM
End Date
20-4-2022 9:05 AM
Abstract
Articular cartilage is a vital connective tissue in the musculoskeletal system. Because articular cartilage is avascular in nature, effective repair of cartilaginous defects remains a challenge. Dimension Inx (Chicago, IL), recently developed a novel ultraporous scaffold: Fluffy polylactide co-glycolide (FPLG). It is capable of being 3D printed to precisely adhere to irregular contours present in cartilaginous surfaces. This study evaluated the chondrogenic potential of FPLG; specifically, its ability to promote viability and adhesion of four immortalized mesenchymal stem cell lines at different stages of differentiation. Immortalized murine embryonic fibroblasts (iMEF), immortalized human urine progenitor cells (iHUP), immortalized murine articular chondrocytes (iMAC), and immortalized multipotent adipose-derived (iMAD) stem cells were infected with recombinant adenovirus GFP and placed onto a 5mm x 5mm x 1mm mesh of FPLG. Cell viability was then measured over 7 days. Fluorescence microscopy imaging showed that the cell lines remained adherent on FPLG, but the cell densities did not increase. Instead they stayed constant for 2 days and decreased thereafter. qPCR analysis demonstrated constant Sox9 expression, an indicator of chondrogenesis. Further tests are warranted to evaluate the chondrogenic potential of FPLG in vitro.
In vitro analysis of an ultraporous synthetic scaffold's ability to promote mesenchymal stem cell survival and chondrogenesis
Articular cartilage is a vital connective tissue in the musculoskeletal system. Because articular cartilage is avascular in nature, effective repair of cartilaginous defects remains a challenge. Dimension Inx (Chicago, IL), recently developed a novel ultraporous scaffold: Fluffy polylactide co-glycolide (FPLG). It is capable of being 3D printed to precisely adhere to irregular contours present in cartilaginous surfaces. This study evaluated the chondrogenic potential of FPLG; specifically, its ability to promote viability and adhesion of four immortalized mesenchymal stem cell lines at different stages of differentiation. Immortalized murine embryonic fibroblasts (iMEF), immortalized human urine progenitor cells (iHUP), immortalized murine articular chondrocytes (iMAC), and immortalized multipotent adipose-derived (iMAD) stem cells were infected with recombinant adenovirus GFP and placed onto a 5mm x 5mm x 1mm mesh of FPLG. Cell viability was then measured over 7 days. Fluorescence microscopy imaging showed that the cell lines remained adherent on FPLG, but the cell densities did not increase. Instead they stayed constant for 2 days and decreased thereafter. qPCR analysis demonstrated constant Sox9 expression, an indicator of chondrogenesis. Further tests are warranted to evaluate the chondrogenic potential of FPLG in vitro.