Event Title

In vitro analysis of an ultraporous synthetic scaffold's ability to promote mesenchymal stem cell survival and chondrogenesis

Session Number

Project ID: MEDH 26

Advisor(s)

Dr. Tong-Chuan He, University of Chicago, Pritzker School of Medicine

Dr. Russell Reid, University of Chicago, Pritzker School of Medicine

Dr. Bryce Hendren-Santiago, University of Chicago, Pritzker School of Medicine

Discipline

Medical and Health Sciences

Start Date

20-4-2022 8:50 AM

End Date

20-4-2022 9:05 AM

Abstract

Articular cartilage is a vital connective tissue in the musculoskeletal system. Because articular cartilage is avascular in nature, effective repair of cartilaginous defects remains a challenge. Dimension Inx (Chicago, IL), recently developed a novel ultraporous scaffold: Fluffy polylactide co-glycolide (FPLG). It is capable of being 3D printed to precisely adhere to irregular contours present in cartilaginous surfaces. This study evaluated the chondrogenic potential of FPLG; specifically, its ability to promote viability and adhesion of four immortalized mesenchymal stem cell lines at different stages of differentiation. Immortalized murine embryonic fibroblasts (iMEF), immortalized human urine progenitor cells (iHUP), immortalized murine articular chondrocytes (iMAC), and immortalized multipotent adipose-derived (iMAD) stem cells were infected with recombinant adenovirus GFP and placed onto a 5mm x 5mm x 1mm mesh of FPLG. Cell viability was then measured over 7 days. Fluorescence microscopy imaging showed that the cell lines remained adherent on FPLG, but the cell densities did not increase. Instead they stayed constant for 2 days and decreased thereafter. qPCR analysis demonstrated constant Sox9 expression, an indicator of chondrogenesis. Further tests are warranted to evaluate the chondrogenic potential of FPLG in vitro.

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Apr 20th, 8:50 AM Apr 20th, 9:05 AM

In vitro analysis of an ultraporous synthetic scaffold's ability to promote mesenchymal stem cell survival and chondrogenesis

Articular cartilage is a vital connective tissue in the musculoskeletal system. Because articular cartilage is avascular in nature, effective repair of cartilaginous defects remains a challenge. Dimension Inx (Chicago, IL), recently developed a novel ultraporous scaffold: Fluffy polylactide co-glycolide (FPLG). It is capable of being 3D printed to precisely adhere to irregular contours present in cartilaginous surfaces. This study evaluated the chondrogenic potential of FPLG; specifically, its ability to promote viability and adhesion of four immortalized mesenchymal stem cell lines at different stages of differentiation. Immortalized murine embryonic fibroblasts (iMEF), immortalized human urine progenitor cells (iHUP), immortalized murine articular chondrocytes (iMAC), and immortalized multipotent adipose-derived (iMAD) stem cells were infected with recombinant adenovirus GFP and placed onto a 5mm x 5mm x 1mm mesh of FPLG. Cell viability was then measured over 7 days. Fluorescence microscopy imaging showed that the cell lines remained adherent on FPLG, but the cell densities did not increase. Instead they stayed constant for 2 days and decreased thereafter. qPCR analysis demonstrated constant Sox9 expression, an indicator of chondrogenesis. Further tests are warranted to evaluate the chondrogenic potential of FPLG in vitro.