Producing a GFP Positive HEK239 Cell Line Via Transduction for In-Vitro Experiments
Session Number
Project ID: MEDH 28
Advisor(s)
Dr. Seth Pollack; Northwestern University, Feinberg School of Medicine
Discipline
Medical and Health Sciences
Start Date
19-4-2023 9:05 AM
End Date
19-4-2023 9:20 AM
Abstract
The Pollack Lab is primarily focused on immunology work with sarcomas, and we recognize the benefits of GFP positive cell lines to image metastases, quantify angiogenesis, and analyze various other hallmarks of cancer and tumor characteristics. Our lab is planning to host a variety of killing assays and in vitro experiments for a new mouse study in which a GFP positive cell-line would be useful. Through this project, I use the following molecular techniques: inoculation of bacteria, transformation, transfection, and transduction alongside live cell imaging. The purpose of my project is to isolate GFP plasmid DNA from Escherichia coli (E. Coli) competent bacterial cells for the transfection of Immortalized Human Embryonic Kidney cells (HEK 293T) to produce GFP virus and subsequently produce a GFP positive cell line via transduction.
Producing a GFP Positive HEK239 Cell Line Via Transduction for In-Vitro Experiments
The Pollack Lab is primarily focused on immunology work with sarcomas, and we recognize the benefits of GFP positive cell lines to image metastases, quantify angiogenesis, and analyze various other hallmarks of cancer and tumor characteristics. Our lab is planning to host a variety of killing assays and in vitro experiments for a new mouse study in which a GFP positive cell-line would be useful. Through this project, I use the following molecular techniques: inoculation of bacteria, transformation, transfection, and transduction alongside live cell imaging. The purpose of my project is to isolate GFP plasmid DNA from Escherichia coli (E. Coli) competent bacterial cells for the transfection of Immortalized Human Embryonic Kidney cells (HEK 293T) to produce GFP virus and subsequently produce a GFP positive cell line via transduction.