Alterations in Osteogenesis Imperfecta: Middle Ear Ossicles and Ligaments During Morphogenesis and Growth
Session Number
Project ID: MEDH 29
Advisor(s)
Dr. Claus-Peter Richter; Northwestern University, Feinberg School of Medicine / Department of Otolaryngology
Discipline
Medical and Health Sciences
Start Date
19-4-2023 9:20 AM
End Date
19-4-2023 9:35 AM
Abstract
Osteogenesis Imperfecta (OI) is a rare collagen disease that affects patients with impaired bone growth, a greater risk of fractures, and other bodily impairments such as hearing loss. This paper observes differences in concentrations of biologically essential elements in the middle ear of oim mice, alendronate-treated oim mice, WT mice, and alendronate-treated WT mice using X-ray fluorescence microscopy (XFM). XFM allows the concentrations of elements within each part of the middle ear to be quantified. The elements measured include iron, calcium, zinc, phosphorus, and copper, and they were selected due to their role in bone growth. Using relative concentration values, the results showed that oim mice obtained larger concentrations in the bone, stapes, and incus compared to the WT mouse across all three comparisons. However, in the malleus, the results differ, the WT mouse showed greater values across all three comparisons. The difference of values between sections of the middle ear is due to the thickness of the samples scanned between the incus, bone, and stapes then with the malleus. Patients with osteogenesis imperfect have lower element concentrations across the elements examined with XFM and draw a correlation to the reason behind why symptoms related to OI occur.
Alterations in Osteogenesis Imperfecta: Middle Ear Ossicles and Ligaments During Morphogenesis and Growth
Osteogenesis Imperfecta (OI) is a rare collagen disease that affects patients with impaired bone growth, a greater risk of fractures, and other bodily impairments such as hearing loss. This paper observes differences in concentrations of biologically essential elements in the middle ear of oim mice, alendronate-treated oim mice, WT mice, and alendronate-treated WT mice using X-ray fluorescence microscopy (XFM). XFM allows the concentrations of elements within each part of the middle ear to be quantified. The elements measured include iron, calcium, zinc, phosphorus, and copper, and they were selected due to their role in bone growth. Using relative concentration values, the results showed that oim mice obtained larger concentrations in the bone, stapes, and incus compared to the WT mouse across all three comparisons. However, in the malleus, the results differ, the WT mouse showed greater values across all three comparisons. The difference of values between sections of the middle ear is due to the thickness of the samples scanned between the incus, bone, and stapes then with the malleus. Patients with osteogenesis imperfect have lower element concentrations across the elements examined with XFM and draw a correlation to the reason behind why symptoms related to OI occur.