Evaluating the degrees of Cerebellar Ataxia on the Genetically Modified PCP2-MW through motor performances and various vivisection techniques

Session Number

Project ID: MEDH 15

Advisor(s)

Dr. Marco Martina; Northwestern University, Feinberg School of Medicine

Dr. Gabriella Sekerkova; Northwestern University, Feinberg School of Medicine

Yen-Hsin Cheng; Northwestern University, Feinberg School of Medicine

Discipline

Medical and Health Sciences

Start Date

19-4-2023 9:35 AM

End Date

19-4-2023 9:50 AM

Abstract

Researchers use a technique called gene targeting, which involves introducing a new gene into the mouse genome and replacing a portion of the endogenous gene with the new gene. In the case of PCP2-MK mice, a modified version of the PCP2 gene is introduced to Purkinje cells in the cerebellum: allowing researchers to study the functions of the cerebellum and its associated neural circuits. The main purpose of this study is to analyze and study the effects of ataxia on this mouse model. Ataxia is a neurological disorder that affects a person's ability to control and coordinate movements. The symptoms of ataxia can vary depending on the underlying cause, but it generally includes: problems with balance and coordination, slurred speech, difficulty with fine motor skills, and tremors. Using techniques such as Rearing, Ledge Test, Footprint Analysis, Rotarod, and Immunostaining, we were able to study the behavioral changes in ataxic mice as well as to study the physical changes made within the brain structure itself. These findings determined a range of degrees in which ataxia can be represented. Determining the behavior and brain function of PCP2 mice may provide insight into the mechanisms underlying ataxia and potential treatments.

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Apr 19th, 9:35 AM Apr 19th, 9:50 AM

Evaluating the degrees of Cerebellar Ataxia on the Genetically Modified PCP2-MW through motor performances and various vivisection techniques

Researchers use a technique called gene targeting, which involves introducing a new gene into the mouse genome and replacing a portion of the endogenous gene with the new gene. In the case of PCP2-MK mice, a modified version of the PCP2 gene is introduced to Purkinje cells in the cerebellum: allowing researchers to study the functions of the cerebellum and its associated neural circuits. The main purpose of this study is to analyze and study the effects of ataxia on this mouse model. Ataxia is a neurological disorder that affects a person's ability to control and coordinate movements. The symptoms of ataxia can vary depending on the underlying cause, but it generally includes: problems with balance and coordination, slurred speech, difficulty with fine motor skills, and tremors. Using techniques such as Rearing, Ledge Test, Footprint Analysis, Rotarod, and Immunostaining, we were able to study the behavioral changes in ataxic mice as well as to study the physical changes made within the brain structure itself. These findings determined a range of degrees in which ataxia can be represented. Determining the behavior and brain function of PCP2 mice may provide insight into the mechanisms underlying ataxia and potential treatments.