Experimental Analysis of the Shinnery Oak Shrub Using High Resolution Unmanned Aerial Vehicle Imagery
Session Number
Project ID: : MEDH 36
Advisor(s)
Dr. Tong Chuan He; University of Chicago
Guozhi Zhao; University of Chicago
Discipline
Medical and Health Sciences
Start Date
19-4-2023 10:35 AM
End Date
19-4-2023 10:50 AM
Abstract
The p53 gene plays a crucial role in regulating the cell cycle and preventing the development of cancer. In renal cancer, the p53 gene is often mutated, leading to uncontrolled cell growth and proliferation. Targeting the p53 gene with gene knockdown techniques has shown promise as a potential treatment for renal cancer. Adenoviruses have been used as a delivery method for gene knockdown in cancer cells, but their effectiveness in clear renal cancer cells has not yet been fully explored. We will use an adenovirus vector carrying a small interfering RNA (siRNA) targeting the p53 gene to deliver the gene knockdown payload to clear renal cancer cells. The siRNA will be designed to specifically bind to the p53 mRNA, leading to its degradation and suppression of p53 protein expression. We will compare the p53 gene knockdown efficiency and downstream effects on cell proliferation in clear renal cancer cells treated with the adenovirus vector to those treated with a control adenovirus vector lacking the siRNA payload.
Experimental Analysis of the Shinnery Oak Shrub Using High Resolution Unmanned Aerial Vehicle Imagery
The p53 gene plays a crucial role in regulating the cell cycle and preventing the development of cancer. In renal cancer, the p53 gene is often mutated, leading to uncontrolled cell growth and proliferation. Targeting the p53 gene with gene knockdown techniques has shown promise as a potential treatment for renal cancer. Adenoviruses have been used as a delivery method for gene knockdown in cancer cells, but their effectiveness in clear renal cancer cells has not yet been fully explored. We will use an adenovirus vector carrying a small interfering RNA (siRNA) targeting the p53 gene to deliver the gene knockdown payload to clear renal cancer cells. The siRNA will be designed to specifically bind to the p53 mRNA, leading to its degradation and suppression of p53 protein expression. We will compare the p53 gene knockdown efficiency and downstream effects on cell proliferation in clear renal cancer cells treated with the adenovirus vector to those treated with a control adenovirus vector lacking the siRNA payload.