Genetic Modification of Adenovirus Vectors for Bone Cancer Treatment
Session Number
Project ID: MEDH 33
Advisor(s)
Tong-Chuan He, Yi Zhu, University of Chicago
Discipline
Medical and Health Sciences
Start Date
17-4-2024 10:00 AM
End Date
17-4-2024 10:15 AM
Abstract
Adenoviruses are frequently employed in clinical studies for oncolytic virotherapy and gene therapy, as well as for delivering genes to different cell types. The adenovirus family consists of non-enveloped DNA viruses with a linear genome of 30–38 kb. The use of human serotype 5 of species C adenovirus vectors is advantageous for delivering genes into a variety of dividing and non-dividing cells both in vitro and in vivo. These vectors allow for high-titer generation and extremely effective gene transfer. Two recombinant adenovirus lines have been constructed. One line contains a green fluorescent protein (GFP), and the other contains a red fluorescent protein (RFP) cassette. This fluorescence helps with precise titer determination. Multiple rounds of cell infection have been started to create a high-titer stock and establish a virus bank that can be used for virus production. The optimal temperatures for virus preservation and virus titer stability will be determined. By doing the above as well as adjusting parameters such as multiplicity of infection, and cell density, stable genes of interest will be effectively and efficiently delivered into mammalian cells by recombinant adenoviruses will be produced.
Genetic Modification of Adenovirus Vectors for Bone Cancer Treatment
Adenoviruses are frequently employed in clinical studies for oncolytic virotherapy and gene therapy, as well as for delivering genes to different cell types. The adenovirus family consists of non-enveloped DNA viruses with a linear genome of 30–38 kb. The use of human serotype 5 of species C adenovirus vectors is advantageous for delivering genes into a variety of dividing and non-dividing cells both in vitro and in vivo. These vectors allow for high-titer generation and extremely effective gene transfer. Two recombinant adenovirus lines have been constructed. One line contains a green fluorescent protein (GFP), and the other contains a red fluorescent protein (RFP) cassette. This fluorescence helps with precise titer determination. Multiple rounds of cell infection have been started to create a high-titer stock and establish a virus bank that can be used for virus production. The optimal temperatures for virus preservation and virus titer stability will be determined. By doing the above as well as adjusting parameters such as multiplicity of infection, and cell density, stable genes of interest will be effectively and efficiently delivered into mammalian cells by recombinant adenoviruses will be produced.