Neonatal Mesechenchymal Stem Cells Seeded Scaffold Support Tissue Regeneration
Session Number
MEDH 08
Advisor(s)
Dr. Arun Sharma, Northwestern University
Stanley Manne Children’s Research Institute
Discipline
Medical and Health Sciences
Start Date
17-4-2024 11:05 AM
End Date
17-4-2024 11:20 AM
Abstract
Bladder exstrophy and neuropathic bladder are conditions that lead to dysfunctional bladder function, which congenital malformations or conditions may cause. Current surgical treatment can lead to a variety of complications, sparking a need for recreating bladder tissue to repair bladder function. During the study utilizing neonatal mesenchymal stem cells (nMSCs)-seeded elastomeric Poly(1,8-octanediol-co-citrate) (POC) scaffolds, rats underwent an approximate 50% bladder cystectomy, then augmented with the nMSC-seeded elastomeric scaffolds. These scaffolds were compared to previously studied adult mesenchymal stem cells (aMSCs)-seeded scaffolds, which showed to improve muscle repair and immune response in the bladder.
Following 4 weeks post augmentation, total bladder capacity was recovered for animals treated with POC-nMSC-seeded bladder scaffolds. This also included non-complications such as kidney stone formation or hydronephrosis. There was a significant regeneration of bladder muscle tissue and percent vasculature of nMSC-seeded scaffolds compared to aMSC scaffolds. Peripheral nerve regeneration was significant in nMSC-seeded scaffolds and absent in aMSC-seeded scaffolds. Peripheral nerve regeneration in the bladder is crucial for proper function, involving sensory nerves signaling bladder fullness and motor nerves coordinating muscle contractions during voiding. nMSC-seeded scaffolds show promise as an alternative treatment to a dysfunctional bladder function.
Neonatal Mesechenchymal Stem Cells Seeded Scaffold Support Tissue Regeneration
Bladder exstrophy and neuropathic bladder are conditions that lead to dysfunctional bladder function, which congenital malformations or conditions may cause. Current surgical treatment can lead to a variety of complications, sparking a need for recreating bladder tissue to repair bladder function. During the study utilizing neonatal mesenchymal stem cells (nMSCs)-seeded elastomeric Poly(1,8-octanediol-co-citrate) (POC) scaffolds, rats underwent an approximate 50% bladder cystectomy, then augmented with the nMSC-seeded elastomeric scaffolds. These scaffolds were compared to previously studied adult mesenchymal stem cells (aMSCs)-seeded scaffolds, which showed to improve muscle repair and immune response in the bladder.
Following 4 weeks post augmentation, total bladder capacity was recovered for animals treated with POC-nMSC-seeded bladder scaffolds. This also included non-complications such as kidney stone formation or hydronephrosis. There was a significant regeneration of bladder muscle tissue and percent vasculature of nMSC-seeded scaffolds compared to aMSC scaffolds. Peripheral nerve regeneration was significant in nMSC-seeded scaffolds and absent in aMSC-seeded scaffolds. Peripheral nerve regeneration in the bladder is crucial for proper function, involving sensory nerves signaling bladder fullness and motor nerves coordinating muscle contractions during voiding. nMSC-seeded scaffolds show promise as an alternative treatment to a dysfunctional bladder function.