Contribution of Periostin to Bone Regeneration
Session Number
2
Advisor(s)
Sarah Cheung, Dr. Frank Ko, Rush University
Location
A119
Discipline
Medical and Health Sciences
Start Date
15-4-2026 11:10 AM
End Date
15-4-2026 11:55 AM
Abstract
Intramembranous bone regeneration is crucial for the healing of orthopedic procedures. Recent studies demonstrate that this is regulated by periostin (Postn). Postn is expressed on the periosteal surface of cortical bone, the dense outer layer of bone. Postn stimulates osteogenesis (bone formation), and its deletion affects Postn-expressing cells (PECs). However, little research has been conducted on the role of periostin and PECs in calvarial defects. To conduct this study, Postn-MCM/+ mice (which have a reduced Postn expression) and Postn-MCM/Postn-MCM mice (which have no Postn expression) were crossed with mice with either the tdTomato gene (fluorescently highlights PECs) or the ROSA-DTA gene (removes all PECs). These mice then underwent a procedure in which a 1.85 mm circular defect on the parietal bone was made. The bone regeneration was then analyzed using microCT and histology. This research demonstrated that Postn deletion impairs healing of calvarial defects, whereas PEC ablation does not, suggesting that these cells do not play a role in bone regeneration.
Contribution of Periostin to Bone Regeneration
A119
Intramembranous bone regeneration is crucial for the healing of orthopedic procedures. Recent studies demonstrate that this is regulated by periostin (Postn). Postn is expressed on the periosteal surface of cortical bone, the dense outer layer of bone. Postn stimulates osteogenesis (bone formation), and its deletion affects Postn-expressing cells (PECs). However, little research has been conducted on the role of periostin and PECs in calvarial defects. To conduct this study, Postn-MCM/+ mice (which have a reduced Postn expression) and Postn-MCM/Postn-MCM mice (which have no Postn expression) were crossed with mice with either the tdTomato gene (fluorescently highlights PECs) or the ROSA-DTA gene (removes all PECs). These mice then underwent a procedure in which a 1.85 mm circular defect on the parietal bone was made. The bone regeneration was then analyzed using microCT and histology. This research demonstrated that Postn deletion impairs healing of calvarial defects, whereas PEC ablation does not, suggesting that these cells do not play a role in bone regeneration.