Investigating the Effects of Torpor on Hematopoietic Stem Cells
Session Number
3
Advisor(s)
Sandra Pinho, PhD, University of Illinois at Chicago; Chandani Patel, M.Sc, University of Illinois at Chicago
Location
A133
Discipline
Chemistry
Start Date
15-4-2026 2:15 PM
End Date
15-4-2026 3:00 PM
Abstract
Torpor and hibernation are responses to harsh environmental conditions such as food scarcity and cold temperatures. Torpor-like states also offer biological advantages such as neuroprotection, radioprotection, and increased lifespan. Space agencies have increased investigations on these topics, hoping to integrate them in future long-term space travel. However, the effects of torpor on mammalian organs and systems are unclear. Here, we use genetic mouse models of torpor to investigate its effects on the hematopoietic system. Hematopoietic stem cells (HSCs) are a rare population in the bone marrow capable of differentiating into all blood cell types, ensuring immune homeostasis. The differentiation and proliferation of HSCs are regulated by their niche, which consists of both hematopoietic and non-hematopoietic cells. Research from the Pinho lab has shown that HSCs are regulated by megakaryocytes. Specifically, megakaryocytes regulate HSC quiescence, a state of dormancy. However, the effects of hibernation-like states on HSCs and niche cells remain unknown and the focus of our studies. Our flow cytometry results revealed that torpor affects HSCs and blocks their proliferation. Cell cycle analysis after torpor showed an increase in quiescent HSCs. Currently, we are using microscopy to investigate effects of torpor on bone marrow megakaryocytes and HSC association with them
Investigating the Effects of Torpor on Hematopoietic Stem Cells
A133
Torpor and hibernation are responses to harsh environmental conditions such as food scarcity and cold temperatures. Torpor-like states also offer biological advantages such as neuroprotection, radioprotection, and increased lifespan. Space agencies have increased investigations on these topics, hoping to integrate them in future long-term space travel. However, the effects of torpor on mammalian organs and systems are unclear. Here, we use genetic mouse models of torpor to investigate its effects on the hematopoietic system. Hematopoietic stem cells (HSCs) are a rare population in the bone marrow capable of differentiating into all blood cell types, ensuring immune homeostasis. The differentiation and proliferation of HSCs are regulated by their niche, which consists of both hematopoietic and non-hematopoietic cells. Research from the Pinho lab has shown that HSCs are regulated by megakaryocytes. Specifically, megakaryocytes regulate HSC quiescence, a state of dormancy. However, the effects of hibernation-like states on HSCs and niche cells remain unknown and the focus of our studies. Our flow cytometry results revealed that torpor affects HSCs and blocks their proliferation. Cell cycle analysis after torpor showed an increase in quiescent HSCs. Currently, we are using microscopy to investigate effects of torpor on bone marrow megakaryocytes and HSC association with them