ER and NFkB activity in BC cell clones vs Bulk population
Session Number
Project ID: MEDH 22
Advisor(s)
Dr. Jonna Frasor; University of Illinois at Chicago
Discipline
Medical and Health Sciences
Start Date
22-4-2020 8:30 AM
End Date
22-4-2020 8:45 AM
Abstract
In order to develop effective treatment to target breast cancer (BC), the relationships between the various molecules involved must be examined. Of these molecules, NFkB (Nuclear Factor kappa B) and ER (Estrogen Receptor) have displayed a possible correlation which could have an effect on breast cancer cells. To analyze the crosstalk between NFkB and ER activity in ER+ BC cells, we examined multiple BC cell line clones transfected with plasmids for NFkB-GFP and ER-mCherry activity to find a clone that represents the bulk population. These cells were then treated with E2 and TNF to activate the plasmids. Cells were scanned for ER and NFkB activity using the Celigo cytometer and analyzed the data based on confluence. This data was plotted and graphed to identify trends between ER/NFkB activity in the clones compared to the activity in the bulk population. Concrete conclusions must be drawn from further results.
ER and NFkB activity in BC cell clones vs Bulk population
In order to develop effective treatment to target breast cancer (BC), the relationships between the various molecules involved must be examined. Of these molecules, NFkB (Nuclear Factor kappa B) and ER (Estrogen Receptor) have displayed a possible correlation which could have an effect on breast cancer cells. To analyze the crosstalk between NFkB and ER activity in ER+ BC cells, we examined multiple BC cell line clones transfected with plasmids for NFkB-GFP and ER-mCherry activity to find a clone that represents the bulk population. These cells were then treated with E2 and TNF to activate the plasmids. Cells were scanned for ER and NFkB activity using the Celigo cytometer and analyzed the data based on confluence. This data was plotted and graphed to identify trends between ER/NFkB activity in the clones compared to the activity in the bulk population. Concrete conclusions must be drawn from further results.